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Arsenite modulation of the integrity of actin filaments and the increase in cell motility may be mediated through CDC42.

Authors
Qian-Y; Zhang-Z; Flynn-DC; Shi-X
Source
FASEB J 2002 Mar; 16(4)(Part I):A171
NIOSHTIC No.
20028371
Abstract
Arsenite is a human carcinogen, however, the molecular mechanisms involved are poorly understood. By stimulating human endothelial cells (SVEC4-10) with arsenite, we found that arsenite modulated the integrity of actin filaments, and stimulated the formation of cell motility organelle structures within 5 minutes. Further, we found arsenite increased the cell motility, indicating that structural changes in cell morphology induced by arsenite are relevant to cellular functions. Affinity absorption assays with GST-PAK-PBD demonstrated that arsenite activated small GTPase CDC42 and Rac. We also observed that arsenite induced the formation of hygrogen radicals in SVEC4-10 cells, which are known to exert a regulatory role in arsenite induced effects on SVEC4-10 cells. Taken together, our data demonstrate that arsenite increases cell motility, which may be involved in cancer matastasis.
Keywords
Arsenites; Carcinogens; Carcinogenicity; Carcinogenesis; Cell-morphology
Contact
PPRB, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
CODEN
FAJOEC
CAS No.
15502-74-6
Publication Date
20020320
Document Type
Conference/Symposia Proceedings; Abstract
Fiscal Year
2002
NTIS Accession No.
NTIS Price
Issue of Publication
4
ISSN
0892-6638
NIOSH Division
HELD
Priority Area
Research Tools and Approaches: Cancer Research Methods
Source Name
The FASEB Journal
State
WV
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