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Oxidative stress and hypersensitivity response to phenolic haptens.

Authors
Shvedova-AA; Kommineni-C
Source
Proceedings of the 12th International Contact Dermatitis Symposium, San Francisco, CA, October 14-18, 1999. Chicago, IL: Skin Disease Education Foundation, 1999 Oct; :9
Link
NIOSHTIC No.
20028179
Abstract
Phenolic haptens undergo oxidative metabolism and produce highly reactive quinone methides that can form conjugates with macromolecules of antigen-presenting cells (APC) and initiate sensitization. One electron oxidation intermediates of phenolic compounds, phenoxyl radicals, can be recycled by different physiological reductants, e.g., antioxidants. We hypothesized that reduction of phenoxyl radicals to their phenolic forms byantioxidants should inhibit/delay formation of quinone methides, and result in abrogation or lessening of the immune response. This effect should depend on the antioxidant/prooxidant status of the APe. We studied oxidation of eugenol (4-allyl-2-methoxyphenol) (EU) to its quinone methide by horseradish peroxidase/H2Oz (HRP/H202) and found that oxidation of EU was effectively inhibited by ascorbate. While dihydrolipoic acid (DHLA) alone was not able to efficiently inhibit oxidation of EU, it interacted synergistically with ascorbate, preventing HRP /Hz02-catalyzed oxidation of ED. In two murine models, we found that oxidation of EU in vivo affected the hypersensitivity response to hapten. Mice sensitized with oxidized EU revealed an enhanced cellular immune response to ED. In mice treated with an antioxidant, lipoic acid (LA), sensitization by EU caused a decreased inflammatory response. We further used vitamin E-deficient mice to test their susceptibility to isoeugenol (IEU)-induced hypersensitivity response. These mice showed decreased levels of vitamin E, GSH and total antioxidant reserves in skin, and elevated levels of mast cell mediated skin responses compared to controls (vitamin E-sufficient mice). We conclude that redox- and antioxidant status of skin are essential determinants of hypersensitivity response to phenolic haptens.
Keywords
Oxidation-reduction-reactions; Oxidation; Oxidative-processes; Antioxidants; Antioxidation; Sensitization; Sensitivity-testing; Laboratory-animals; Animal-studies; Phenols; Phenolic-compounds; Phenolic-acids
Publication Date
19991001
Document Type
Abstract; Conference/Symposia Proceedings
Fiscal Year
2000
NTIS Accession No.
NTIS Price
NIOSH Division
HELD
Source Name
Proceedings of the 12th International Contact Dermatitis Symposium, San Francisco, CA, October 14-18, 1999
State
WV; CA
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