Changes in F-actin organization induced by hard metal particle exposure in rat pulmonary epithelial cells as observed by laser scanning confocal microscopy.
Antonini-JM; Starks-K; Roberts-JR; Millecchia-LL; Yang-HM; Rao-KMK
Microsc Microanal 1999 Aug; 5(Suppl 2):492-493
Hard metal is an alloy of tungsten carbide and cobalt along with other components such as chromium carbide, molybdenum carbide, tantalum carbide, and nickel. Chronic exposure to hard metal particles by inhalation causes alveolitis leading to interstitial fibrosis, the pathogenesis of which is still undefined. The initial inflammatory response includes a change in epithelial cell permeability barrier function (1) which has been shown to be regulated by the state of assembly and organization of the actin cytoskeletal network (2, 3). Therefore, the objective of this study was to evaluate the effect hard metal particles have on F-actin organization of rat lung epithelial cells in an in vitro culture system.
Exposure-levels; Laboratory-animals; Animals; Animal-studies; Pulmonary-system-disorders; Inhalation-studies; Fibrosis; Lung-cells; Metals; Lung-fibrosis; Respiratory-system-disorders; Heavy-metals; Heavy-metal-poisoning; Metal-poisoning; Metal-compounds; Metal-dusts
7440-48-4; 7439-98-7; 7440-02-0
Microscopy and Microanalysis