Toughness of skin in arsenic exposed mice: role of GM-CSF and TGFa.
Yoshida-T; Aikawa-H; Yamauchi-H; Sakabe-K; Kayama-F; Fujimoto-W; Germolec-DR; Luster-MI
Toxicologist 1999 Mar; 48(1-S):353
Hyperkeratosis on the palms of the hand and soles of the feet are characteristics of arsenic (As) poisoning. However, toughness of skin has not been described as a manifestation of As poisoning. We previously reported that As induces GM-CSF and TGFa mRNA and protein production in human keratinocyte culture. In the present studies sodium arsenite was provided at 1 and 10mg/L to C57B/6n male and female mice via drinking water for 50 days. As concentrations were determined in hair and urine. Pieces of skin were excised from shaved ventral skin by scissors for histpathological and molecular analysis. Increase skin toughness dominantly in male was apparent in exposed groups when skin sections were prepared. Skin sections were prepared and stained for Haematoxilin and Eosin. GM-CSF in serum were measured by ELISA. RNA was extracted from skin and the expression of GM-CSF and TGFa mRNA were examined by RT-PCR. Although the epidermis appeared normal, the dermis in exposed animals showed moderate thickening of the collagen bundles. Seven of 12 exposed male mice and 4 of 12 female mice showed significantly thickened collagen bundles. GM-CSF in serum were elevated in 10mg/L, but not significantly. RNA expressions for GM-CSF and TGFa were enhanced moderately in exposed groups compared to controls. These results indicate that a number of changes occur in the skin of As-exposed mice, including thickening of collagen bundles and increased growth factor expression.
Toxins; Toxicology; Toxic-materials; Laboratory-animals; Animal-studies; Statistical-analysis; Analytical-chemistry; Analytical-methods; Chemical-analysis; Dose-response; Exposure-assessment; Sensitivity-testing; Sensitization; Arsenic-poisoning; Arsenic-compounds; Skin-exposure; Skin-disorders; Skin-irritants; Skin-tests; Skin-sensitivity
The Toxicologist. Society of Toxicology 38th Annual Meeting, March 14-18, 1999, New Orleans, Louisiana