The influence of (1-->3)Beta-D glucan on endotoxin-induced acute pulmonary inflammation.
Roy-CJ; O'Neill-ME; Thorne-PS
Toxicologist 1999 Mar; 48(1-S):133
Organic dust aerosols, known to cause respiratory disease among fanners, are composed of biogenic agents including bacteria, fungi, aeroallergans, excreta, dander, bacterial endotoxins (Etx) and glucans. Sclerotinia sclerotiorum (85) is an emerging phytopathogenic soybean fungus rich in highly branched, water soluble (1-->3)Beta-D glucans to which farmers are exposed. After a first study revealed reduced Etx-induced pulmonary inflammation in mice instilled with Ss extract and Etx, experiments were performed to assess the influence of Ss glucan content in the blunting of Etx-induced inflammation. BALB/cJ mice were intratracheally instilled with SO 11 g of purified scleroglucan (GL) alone, SO microg of Ss alone; or SO microg of GL or Ss in combination with 0.25 microg of Etx, or saline (control). Bronchoalveolar lavage (BAL) collected 4 hr postexposure from the Etx group showed an increase in total HAL cells (96% neutrophils) with an increase of MIP-2, TNF-alpha and IL-6 over controls. GL or Ss exposure alone did not induce changes in HAL cellularity or MIP-2 concentration when compared to, controls. However, a reduction of 010 BAL neutrophils (p<0.01) was observed in the GL+Etx group when compared with the Etx group, although total BAL cells remained elevated. The neutrophil response to the Ss+Etx group was similar to the GL+Etx group (p<0.01), and total BAL cells were significantly reduced when compared to the Etx group (p<0.01). These results suggest that an antagonistic dose-response relationship exists for concomitant GL+Etx exposures and the soluble glucans in S. sclerotiorum may be responsible for the reduction of pulmonary inflammation.
Toxicology; Toxins; Toxic-materials; Statistical-analysis; Analytical-methods; Analytical-chemistry; Chemical-analysis; Animal-studies; Laboratory-animals; Exposure-assessment; Airborne-particles; Particulates; Dust-particles; Dust-exposure; Farmers; Respiratory-system-disorders; Pulmonary-system-disorders; Neutrophils
The Toxicologist. Society of Toxicology 38th Annual Meeting, March 14-18, 1999, New Orleans, Louisiana
University of Iowa, Iowa City, Iowa