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Induction of stress proteins in rat cardiac myocytes by antimony.

Authors
Snawder-JE; Tirmenstein-MA; Mathias-PI; Toraason-M
Source
Toxicol Appl Pharmacol 1999 Sep; 159(2):91-97
NIOSHTIC No.
20027721
Abstract
The effects of nonlethal concentrations of potassium antimonyl tartrate (PAT) were examined in cultured neonatal rat cardiac myocytes. PAT (5, 10 mM) significantly increased cellular reduced glutathione (GSH) and heme oxygenase activity after 18 h. GSH levels and heme oxygenase activity were increased 2.5- and 5.4- fold, respectively, by 10 mM PAT after 18 h. In addition, total cytochrome P450 levels were decreased by PAT after an 18-h exposure. PAT exposures were associated with the induction of specific stress proteins. Nonlethal concentrations of PAT produced a dose-dependent increase in HO-1, HSP70, and HSP25/27 protein levels but did not increase HSP60 levels. Pretreatment of cardiac myocytes with low concentrations of PAT (0.5-10 mM) protected against a subsequent lethal concentration of PAT (200 mM). This protection was blocked if cells were treated with the protein synthesis inhibitor cycloheximide. Results demonstrate that low concentrations of PAT increase GSH levels and stress protein synthesis, which may be responsible for the protection that low level PAT exposure offers against the subsequent toxicity of higher concentrations of PAT.
Keywords
Antimony-compounds; Myocardium; Myocardial-disorders; Animal-studies; Animals; Laboratory-animals; Laboratory-testing; Cardiac-function; Heart; Potassium-compounds; Stress; Cardiovascular-system-disorders; Cardiopulmonary-system-disorders; Statistical-analysis; Analytical-chemistry; Analytical-methods
Contact
Mark Toraason, Cellular Toxicology Section, National Institute for Occupational Safety and Health, 4676 Columbia Parkway, Cincinnati, Ohio 45226
CODEN
TXAPA9
Publication Date
19990901
Document Type
Journal Article
Fiscal Year
1999
NTIS Accession No.
NTIS Price
Issue of Publication
2
ISSN
0041-008X
NIOSH Division
DBBS
Source Name
Toxicology and Applied Pharmacology
State
OH
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