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Effect of magnetic field exposure on anchorage-independent growth of a promoter-sensitive mouse epidermal cell line (JB6).

Authors
Snawder-JE; Edwards-RM; Conover-DL; Lotz-WG
Source
Environ Health Perspect 1999 Mar; 107(3):195-198
NIOSHTIC No.
20027720
Abstract
The anchorage-independent growth of mouse epidermal cells (JB6) exposed to 60-Hz magnetic fields (MF) was investigated. Promotion-responsive JB6 cells were suspended in agar (104 cells/plate) and exposed continuously to 0.10 or 0.96 mT, 60-Hz magnetic fields for 10-14 days, with or without concurrent treatment with the tumor promoter tetradecanoylphorbol acetate (TPA). Exposures to MF were conducted in a manner such that the experimenter was blind to the treatment group of the cells. At the end of the exposure period, the anchorage-independent growth of JB6 cells on soft agar was examined by counting the number of colonies larger than 60 Ám (minimum of 60 cells). The use of a combined treatment of the cells with both MF and TPA was to provide an internal positive control to estimate the success of the assay and to allow evaluation of co-promotion. Statistical analysis was performed by a randomized block design analysis of variance to examine both the effect of TPA treatment (alone and in combination with MF exposure) and the effect of intra-assay variability. Transformation frequency of JB6 cells displayed a dose-dependent response to increasing concentrations of TPA. Coexposure of cells to both TPA and 0.10 or 0.96 mT, 60-Hz MF did not result in any differences in transformation frequency for any TPA concentrations tested (0-1 ng/ml). These data indicate that exposure to a 0.10 or 0.96 mT, 60-Hz MF does not act as a promoter or co-promoter in promotion-sensitive JB6 cell anchorage-independent growth.
Keywords
Magnetic-fields; Magnetic-properties; Statistical-analysis; Cancer; Epidemiology; Animal-studies; Animals; Laboratory-animals; Cell-cultures; Cell-damage; Cell-function
Contact
J.E. Snawder, Taft Laboratories, MS-C23, National Institute for Occupational Safety and Health, 4676 Columbia Parkway, Cincinnati, OH 45226 USA
CODEN
EVHPAZ
Publication Date
19990301
Document Type
Journal Article
Fiscal Year
1999
NTIS Accession No.
NTIS Price
Issue of Publication
3
ISSN
0091-6765
NIOSH Division
DBBS
Source Name
Environmental Health Perspectives
State
OH
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