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Neoplastic potential of rat tracheal epithelial cell lines induced by 1-nitropyrene and dibenzo(a,i)pyrene.

Authors
Ensell-MX; Hubbs-A; Zhou-G; Batelli-L; Nath-J; Ong-T
Source
Mutat Res 1999 Jul; 444(1):193-199
NIOSHTIC No.
20027683
Abstract
Our previous study showed that both 1-nitropyrene (1-NP) and dibenzo(a,i)pyrene (DBP) induced enhanced growth variants (EGVs) in primary cultures of rat tracheal epithelial (RTE) cells exposed in vivo. Cell lines were established from some of the EGVs. Further studies, using anchorage-independent growth in soft agar and tumorigenicity in athymic nude mice, were performed to determine the neoplastic potential of EGVs induced by 1-NP and DBP. Results show that three of five from DBP- and five of five from 1-NP-induced cell lines displayed anchorage-independent growth. The colony forming efficiency (CFE) from DBP-induced cell lines was 0.067 per thousand and CFE from 1-NP-induced cell lines was 0.151 per thousand. There is a significant difference between the two CFEs (mu = 12.08, P<0. 01). Two of five DBP- and five of five 1-NP-induced cell lines produced squamous cell carcinomas (SCC) in nude mice. The rate of tumorigenicity counted by injected sites was 20% (6/30) for DBP-induced cell lines and 57% (17/30) for 1-NP-induced cell lines. There is a significant difference between the results of tumorigenicity from the cell lines induced by the two different compounds (chi(2)=8.53, P<0.01). Neither of the two cell lines from spontaneously developed foci grew in soft agar or produced SCC in nude mice. It seems that the neoplastic potential of transformed RTE cells induced by 1-NP was higher than that of DBP.
Keywords
Animal-studies; Animals; Neoplastic-agents; Statistical-analysis; Analytical-methods; Analytical-models; Analytical-processes; Benzopyrenes; Carcinogenicity; Carcinogens; Toxic-materials; Cell-division; Cell-transformation
Contact
Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, MS 3014, 1095 Willowdale Road, Morgantown, WV 26505
CODEN
MUREAV
CAS No.
189-55-9; 5522-43-0
Publication Date
19990721
Document Type
Journal Article
Email Address
too2@cdc.gov
Fiscal Year
1999
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
0027-5107
NIOSH Division
HELD
Source Name
Mutation Research
State
WV
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