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Determination of benzo[a]pyrene-DNA adducts by solid-matrix phosphorescence.

Authors
Li-M; Hurtubise-RJ; Weston-A
Source
Anal Chem 1999 Oct; 71(20):4679-4683
NIOSHTIC No.
20027323
Abstract
It has been demonstrated for the first time that linear relationships can be obtained between the solid-matrix phosphorescence (SMP) and the percent modification of benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE)-DNA adducts. The samples of DNA were modified with BPDE at levels of 5.0 10-3, 1.0 10-3, 5.0 10-4, 1.0 10-4, 5.0 10-5, and 1.0 10-5%. In addition, the changes in the SMP intensities of a given percentage of DNA adduct were investigated as a function of sample size, and linear relationships were also acquired. With the different percentages of modified DNA, very good reproducibility of the SMP signals was obtained. Data were acquired with both Whatman 1PS paper and 30% TlNO3/sodium acetate as solid matrixes. The limit of detection for the BPDE-DNA adducts was 2 adducts in 107 bases for both Whatman 1PS paper and 30% TlNO3/sodium acetate. In addition, it was shown that it would be important to develop a standard procedure for the preparation of the BPDE-DNA samples if different batches of DNA were used in the preparation procedure.
Keywords
DNA-adducts; Phosphorescence-polarization; Sampling; Sampling-methods; Sodium-compounds
Contact
NIOSH/CDC/HELD/TMBB, 1095 Willowdale Road, MS 3014, Morgantown, West Virginia 26505
CODEN
ANCHAM
CAS No.
13345-25-0; 127-09-3
Publication Date
19991001
Document Type
Journal Article
Fiscal Year
2000
NTIS Accession No.
NTIS Price
Issue of Publication
20
ISSN
0003-2700
NIOSH Division
HELD
Source Name
Analytical Chemistry
State
WV; NJ
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