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Application of animal models for immediate and delayed pulmonary hypersensitivity: characteristics of delayed reactions to tuberculin protein.

Karol-MH; Magreni-C; Stadler-J
Proceedings of the Thirteenth Conference on Environmental Toxicology, Dayton, Ohio, November 16-18, 1982. Dayton, Ohio: Air Force Aerospace Medical Research Laboratory, 1983 Aug; :80-87
A method was described recently for monitoring delayed-onset pulmonary hypersensitivity reactions in guinea pigs (Karol et al., 1981) . To induce sensitivity, animals were injected with Freud's complete adjuvant. Pulmonary reactivity was demonstrated three to five weeks later by bronchial provocation challenge with aerosolized tuberculin protein derivative (PPD). Because onset of reaction occurred typically 9-12 hr following challenge, it was necessary to challenge animals in the "heads-only" inhalation chamber, then remove them from the chamber immediately following challenge and place animals in individual whole body plethysmographs for long-term monitoring. In this way, guinea pigs were free to move about during the ensuing 24 hr while respiratory responses were being monitored. However, the transfer of animals from the "heads-only" to the whole body plethysmographs resulted in loss of measurements for about 1 hr. In order to provide continuous measurements of respiratory rate, beginning prior to challenge and continuing for 24, a system was established based on a recent report (Zelenak et al., 1982). The current study describes characteristics of the continuous respiratory response to PPD in guinea pigs. In addition, experiments were performed to determine the influence of several factors on the pattern of delayed-onset responses. Such factors included: repeated inhalation challenge with homologous antigen, time interval between challenges, and history of previous pulmonary reactions to heterologous antigens. Results indicated that respiratory responses to PPD were influenced by previous exposure only to homologous antigen.
Animals; Animal-studies; Pulmonary-disorders; Pulmonary-function; Pulmonary-function-tests; Pulmonary-system; Pulmonary-system-disorders; Inhalation-studies; Respiratory-system-disorders; Respiratory-irritants; Respiratory-hypersensitivity; Respiratory-function-tests; Aerosols; Antigens; Exposure-methods; Exposure-chambers
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Conference/Symposia Proceedings
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Proceedings of the Thirteenth Conference on Environmental Toxicology, Dayton, Ohio, November 16-18, 1982
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University of Pittsburgh