Micronucleus induction and DNA damage in V 79 cells in vitro by dusts from hard metal sintering and detonation coating processes.
Toxicologist 2005 Mar; 84(Suppl 1):455-456
Hard metal bulk dusts from a molding-sintering process and from a detonation-coating process were characterized for their ability to induce micronuclei and DNA damage in cultured V 79 Chinese hamster lung fibroblast cells. The study included the precoating mixture and overspray material from a detonation coating process, an unsintered material from a molding-sintering operation, and the tungsten carbide and cobalt ingredients from the sintering process. Doses were selected after viability testing and ranged from 0 to 200 ?g/cm2. Results indicate that the unsintered sample and pre- and post-coating detonation coating mixtures showed a positive dose-response relationship for micronucleus induction; WC alone was weakly positive for a single dose only. The overspray material from the detonation-coating process showed greater micronucleus induction than any of the other materials. Results from the DNA damage assay indicate that the three hard metal mixtures are all capable of DNA damage, with similar dose-response curves. The addition of 10 or 20 mM N-acetyl cysteine, a general antioxidant, significantly attenuated DNA damage by all three samples. The results indicate that materials from either conventional or detonation-coating processes are capable of genotoxicity in V 79 cells in vitro, including DNA damage and micronucleus induction, and DNA damage is at least partially through oxidative mechanisms. The post-detonation material is a more potent inducer of micronuclei than are the predetonation and unsintered dusts, but the DNA damage potential of all three dusts are quantitatively similar.
DNA-damage; In-vitro-studies; Dusts; Metal-dusts; Metal-compounds; Coatings; Laboratory-animals; Animals; Animal-studies; Lung-cells; Dose-response; Sampling; Quantitative-analysis; Mutagenicity
Work Environment and Workforce: Mixed Exposures
The Toxicologist. Society of Toxicology 44th Annual Meeting and ToxExpo, March 6-10, 2005, New Orleans, Louisiana