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Free radical scavenging, DNA protection, and inhibition of lipid peroxidation mediated by uric acid.

Authors
Stinefelt-B; Leonard-SS; Blemings-KP; Shi-X; Klandorf-H
Source
Ann Clin Lab Sci 2005 Jan; 35(1):37-45
NIOSHTIC No.
20026395
Abstract
Uric acid (UA) has been proposed to be the dominant antioxidant in birds. The objective of this study was to investigate the quenching effect of varying concentrations of UA, including those found in avian plasma, on specific reactive oxygen species (ROS) and to determine the ability of UA to protect DNA and cellular membranes from ROS-mediated damage. Hydroxyl (OH) and superoxide (O2) radicals were detected by electron spin resonance (ESR) and their presence was reduced following addition of UA (p <0.05) in a concentration-dependent manner. UA inhibited hydroxyl-mediated DNA damage, indicated by the presence of more precise, dense bands of Hind III DNA after agarose gel electrophoresis and ethidium bromide staining (p <0.05). Lipid peroxidation of silica-exposed RAW 264.7 cell membranes was diminished (p <0.02) after addition of UA to the cell incubation mixture. These studies demonstrate that UA scavenges hydroxyl and superoxide radicals and protects against DNA damage and lipid peroxidation. These results indicate specific antioxidant protection that UA may afford birds against ROS-mediated damage.
Keywords
Free-radicals; Free-radical-generation; Lipids; Lipid-peroxidation; Antioxidants; DNA-damage; Hydroxyl-groups; Silica-dusts; Silicates; Exposure-levels
Contact
Kenneth P. Blemings, Ph.D., West Virginia University, P.O. Box 6108, Morgantown, WV 26506, USA
CODEN
ACLSCP
Publication Date
20050101
Document Type
Journal Article
Email Address
kbleming@wvu.edu
Fiscal Year
2005
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
0091-7370
NIOSH Division
HELD
Priority Area
Research Tools and Approaches: Cancer Research Methods
Source Name
Annals of Clinical and Laboratory Science
State
WV
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