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Elevated oxidative stress in skin of B6C3FL mice affects dermal exposure to machine working fluid.

Kommineni-CV; Kisin-E; Al-Humadi-N; Castranova-V; Shvedova-AA
Toxicologist 2000 Mar; 54(1):141
Metal working fluids (MWFs) are widely used for metal cutting, drilling, shaping, lubricating and milling in the industrial world. Potential for dermal and inhalation exposure to MWFs exists in a large number of worker via aerosols, splashing during the machining process, and other industrial operations. Both females and males are involved in these industrial operations. It has been reported earlier that occupational exposure to MWFs causes health problems, e.g. allergic and irritant contact dermatitis. Previously, we have shown that dermal exposure of B6C3F1 mice (female and male) to 5% MWFs for 3 months resulted in depletion of two major water soluble antioxidants, e.g. ascorbate and glutathione in the liver of both genders. The level of lipid peroxidation (MDA) in the liver of both sexes and the testes in males was concomitantly increased. The goal of this study was to evaluate interaction between oxidative stress in the skin and topical application of MWF. To create acute skin inflammation, an H2O2 producing enzyme, glucose oxidase conjugated to polyethylene glycol (GOD-PEG), was injected intradermally. Intradermal administration of GOD-PEG to B6C3F1 mice (female and male, 8-12 weeks old) resulted in inflammation and muscle fiber necrosis in the skin. The level of GSH in skin was reduced after GOD-PEG treatment of B6C3F1 mice. To determine whether oxidative stress influences the dermal response to MWF, mice were injected with GOD-PEG 24 h prior to topical exposure to unused MWF (200 ul 30%). This resulted in an increased inflammatory response in skin, reduced GSH and protein thiols, and an increased level of peroxidative products. Further study is necessary to delineate the role of oxidative stress in enhancing contact/irritant dermatitis triggered by occupational exposures to MWF.
Metalworking-fluids; Metalworking; Metalworking-industry; Inhalation-studies; Workers; Aerosols; Occupational-exposure; Exposure-levels; Machinists; Machine-operators; Demographic-characteristics; Sex-factors; Health-hazards; Allergic-dermatitis; Contact-dermatitis; Dermatitis; Laboratory-animals; Animals; Animal-studies; Skin-irritants
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The Toxicologist. Society of Toxicology 39th Annual Meeting, March 19-23, 2000, Philadelphia, Pennsylvania