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Intracellular behavior of beryllium oxide particles: an in vitro study in murine macrophages.

Authors
Day-GA; Stefaniak-AB; Hoover-MD; Dickerson-RM; Peterson-EJ; Esman-NA; Scripsick-RC
Source
Am J Respir Crit Care Med 2004 Apr; 169(7)(Abstracts):A640
NIOSHTIC No.
20025897
Abstract
Beryllium metal, its oxide (BeO), and alloys are materials of industrial significance with recognized adverse effects on worker health. Currently, the degree of risk associated with exposure to these materials in the workplace is assessed through measurement of beryllium aerosol mass concentration, the control of which has proven ineffective at eliminating the occurrence of chronic beryllium disease. The rationale for this research was to examine the role of bioavailability, rather than mass alone, as a key factor for risk. Physicochemical changes were evaluated in vitro for well-characterized high-purity BeO particles engulfed by and retained witl2in murine J774A.1 monocyte-macrophage cells. The BeO was a commercially available powder consisting of diffuse clusters of 200-nm diameter primary particles. Following incubation for 124 to 144 hours, engulfed particles were recovered for re-characterization. Recovered particles were similar in morphology, chemical composition, and size to the original material, confirming the insoluble nature of the BeO particles. Measurable levels of dissolved beryllium, representing 0.3 to 4.8% of the estimated total beryllium mass added, were found in the recovered intracellular fluid; dissolved beryllium was not found in the extracellular fluid. Release of beryllium may occur in tl2e alveolar environment after macrophage death, in the lymphatic system after transport of beryllium by macrophages, or in the alveolar interstitium after migration and slower dissolution of beryllium particles in tissue. These findings demonstrate the bioavailability of dissolved beryllium, and are consistent with previously observed results in canine alveolar macrophages
Keywords
Beryllium-disease; Beryllium-poisoning; Beryllium-compounds; Metal-oxides; Metal-poisoning; Copper-alloys; Aerosols; Aerosol-particles; Airborne-particles; Airborne-dusts; Metal-compounds; Metal-dusts; Metal-fumes; Metal-industry; Metal-industry-workers; Metal-workers; Metallic-compounds; Metallic-dusts; Metallic-fumes; Metallic-poisoning; Metallic-poisons; Metals; Pulmonary-system-disorders; Pulmonary-disorders; Respirable-dust; Respiratory-irritants; Respiratory-system-disorders;
CODEN
AJCMED
CAS No.
7440-41-7; 7440-50-8
Publication Date
20040401
Document Type
Conference/Symposia Proceedings; Abstract
Email Address
gday@cdc.gov
Funding Amount
76780
Funding Type
Grant
Fiscal Year
2004
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R03-OH-007447
Issue of Publication
7
ISSN
1073-449X
NIOSH Division
DRDS
Priority Area
Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
Source Name
American Journal of Respiratory and Critical Care Medicine
State
NM; MD; WV; OK; OH
Performing Organization
Johns Hopkins University, Baltimore, Maryland
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