Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

The sources of inflammatory mediators in the lung after silica exposure.

Authors
Rao-KM; Porter-DW; Meighan-T; Castranova-V
Source
Environ Health Perspect 2004 Dec; 112(17):1679-1686
NIOSHTIC No.
20025841
Abstract
The expression of 10 genes implicated in regulation of the inflammatory processes in the lung was studied after exposure of alveolar macrophages (AMs) to silica in vitro or in vivo. Exposure of AMs to silica in vitro up-regulated the messenger RNA (mRNA) levels of three genes [interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and macrophage inflammatory protein-2 (MIP-2)] without a concomitant increase in the protein levels. AMs isolated after intratracheal instillation of silica up-regulated mRNA levels of four additional genes [granulocyte/macrophage-colony stimulating factor (GM-CSF), IL-1beta, IL-10, and inducible nitric oxide synthase]. IL-6, MCP-1, and MIP-2 protein levels were elevated in bronchoalveolar lavage fluid. Fibroblasts under basal culture conditions express much higher levels of IL-6 and GM-CSF compared with AMs. Coculture of AMs and alveolar type II cells, or coculture of AMs and lung fibroblasts, in contact cultures or Transwell chambers, revealed no synergistic effect. Therefore, such interaction does not explain the effects seen in vivo. Identification of the intercellular communication in vivo is still unresolved. However, fibroblasts appear to be an important source of inflammatory mediators in the lung.
Keywords
Silica-dusts; Silicates; Exposure-levels; Genes; Lung-irritants; In-vitro-studies; In-vivo-studies
Contact
K.M.K. Rao, Box 2015, PPRB/HELD/NIOSH, 1095 Willowdale Road, Morgantown, WV 26505 USA
CODEN
EVHPAZ
CAS No.
14808-60-7
Publication Date
20041201
Document Type
Journal Article
Email Address
mir8@cdc.gov
Fiscal Year
2005
NTIS Accession No.
NTIS Price
Issue of Publication
17
ISSN
0091-6765
NIOSH Division
HELD
Source Name
Environmental Health Perspectives
State
WV
TOP