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Characterization of phagolysosomal simulant fluid for study of beryllium aerosol particle dissolution.

Authors
Stefaniak-AB; Guilmette-RA; Day-GA; Hoover-MD; Breysse-PN; Scripsick-RC
Source
Toxicol In Vitro 2005 Feb; 19(1):123-134
NIOSHTIC No.
20025800
Abstract
A simulant of phagolysosomal fluid is needed for beryllium particle dissolution research because intraphagolysosomal dissolution is believed to be a necessary step in the cellular immune response associated with development of chronic beryllium disease. Thus, we refined and characterized a potassium hydrogen phthalate (KHP) buffered solution with pH 4.55, termed phagolysosomal simulant fluid (PSF), for use in a static dissolution technique. To characterize the simulant, beryllium dissolution in PSF was compared to dissolution in the J774A.1 murine cell line. The effects of ionic composition, buffer strength, and the presence of the antifungal agent alkylbenzyldimethylammonium chloride (ABDC) on beryllium dissolution in PSF were evaluated. Beryllium dissolution in PSF was not different from dissolution in the J774A.1 murine cell line (p=0.78) or from dissolution in another simulant having the same pH but different ionic composition (p=0.73). A buffer concentration of 0.01-M KHP did not appear adequate to maintain pH under all conditions. There was no difference between dissolution in PSF with 0.01-M KHP and 0.02-M KHP (p=0.12). At 0.04-M KHP, beryllium dissolution was increased relative to 0.02-M KHP (p=0.02). Use of a 0.02-M KHP buffer concentration in the standard formulation for PSF provided stability in pH without alteration of the dissolution rate. The presence of ABDC did not influence beryllium dissolution in PSF (p=0.35). PSF appears to be a useful and appropriate model of in vitro beryllium dissolution when using a static dissolution technique. In addition, the critical approach used to evaluate and adjust the composition of PSF may serve as a framework for characterizing PSF to study dissolution of other metal and oxide particles.
Keywords
Beryllium-compounds; Beryllium-disease; Physiological-chemistry; Stimulants; Cellular-reactions; Aerosol-particles; Respiratory-system-disorders; Phagocytic-activity; In-vitro-studies; Immune-reaction; Ammonium-compounds; Alveolar-cells; Chlorides; Phthalates; Potassium-compounds; Phosphates; Oxides; Author Keywords: Alveolar macrophage phagolysosome; Dissolution; Simulant; Chronic beryllium disease
Contact
A.B. Stefaniak, National Institute for Occupational Safety and Health, Division of Respiratory Disease Studies, 1095 Willowdale Road, Morgantown, WV 26505
CODEN
TIVIEQ
CAS No.
7440-41-7; 1304-56-9; 877-24-7
Publication Date
20050201
Document Type
Journal Article
Email Address
astefaniak@cdc.gov
Funding Amount
76780
Funding Type
Grant
Fiscal Year
2005
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R03-OH-007447; Grant-Number-T42-OH-008428
Issue of Publication
1
ISSN
0887-2333
NIOSH Division
DRDS
Priority Area
Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
Source Name
Toxicology in Vitro
State
MD; NM; WV
Performing Organization
Johns Hopkins University, Baltimore, Maryland
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