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Immunomodulation of the IGE response to natural rubber latex (NRL) proteins by endotoxin and glutaraldehyde.

Authors
Howell-MD; Tomazic-Jezic-V; Leakakos-T; Truscott-W; Johnson-CM; Meade-BJ
Source
Toxicologist 2001 Mar; 60(1):174-175
NIOSHTIC No.
20025780
Abstract
Reduction of the protein and powder content of NRL gloves has been recommended to reduce the incidence of latex sensitization and symptoms associated with NRL exposure. A better understanding of the effects of co-exposure with other irritant/sensitizers on the development of NRL allergy will be helpful in developing effective intervention strategies. These studies were conducted to investigate the effect of co-exposure to the common health care contaminants endotoxin and glutaraldehyde on the development of an IgE response to NRL. Endotoxin was tested at 50 -25,000 EU while concentrations of glutaraldehyde tested were representative of the concentrations found in cold sterilant solutions (0.1 - 1 %) and aerosol levels surrounding the permissible exposure limits (PEL, 0.05 - 1 ppm). Female BALB/c mice were concurrently exposed to endotoxin (intranasally, subcutaneously) or glutaraldehyde (dermally) and 25 microg of NRL proteins 5 days a week for up to 17 weeks. Mice were tail bled prior to exposure and bi-weekly thereafter to obtain total and specific serum immunoglobulin levels. Animals exposed concurrently to endotoxin and NRL exhibited a dose dependent decrease in total and NRL specific serum IgE levels reaching >50% suppression and increase in serum IgG1 and IgG2a levels. Mice exposed to glutaraldehyde in concentrations consistent with cold sterilant solutions exhibited decreases in total (>50%) or NRL specific IgE levels when compared to mice exposed to NRL alone. However, upon co-exposure to levels of glutaraldehyde in the range of the PEL a dose responsive increase in both total (3 fold) and NRL specific (>40 fold) IgE was observed. These studies demonstrated that endotoxin induced an immunoglobulin class switch whereas glutaraldehyde augmented the IgE response to NRL proteins.
Keywords
Endotoxins; Sensitization; Irritants; Allergic-reactions; Health-care; Exposure-assessment; Exposure-limits; Aerosols; Laboratory-animals; Animals; Animal-studies
CAS No.
111-30-8
Publication Date
20010301
Document Type
Abstract
Fiscal Year
2001
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
1096-6080
NIOSH Division
HELD
Source Name
The Toxicologist. Society of Toxicology 40th Annual Meeting, March 25-29, 2001, San Francisco, California
State
WV; DC; CA
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