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Restraint stress modifies Langerhans cell migration and lymph node cell proliferation.

Authors
Flint-MS; Abrigo-BA; Klenner-JW; Tinkle-SS
Source
Toxicologist 2001 Mar; 60(1):171
NIOSHTIC No.
20025759
Abstract
Previously we have demonstrated that restraint applied to a naive mouse prior to sensitization decreases ear swelling and restraint applied prior to challenge increased ear swelling. We hypothesize that these dichotomous effects of restraint would be reflected in changes in LC trafficking from the epidermis or alteration of their antigen presenting capabilities to T cells in the draining lymph nodes. To assess the effect of restraint on T cell proliferation in vivo, male BALB/c mice were exposed on the dorsum of both ears on days 1, 2 and 3 with 0.25% di-nitrofluorobenzene (DNFB; n = 5) or vehicle only, and restrained for 2 hours prior to chemical application on day 1. On day 5, T lymphocyte proliferation was examined using the local lymph node assay. To evaluate the effects of restraint on LC, mice were sensitized with 0.25% DNFB immediately after 2 hour restraint. LC morphology and migration were examined in epidermal sheets by counting the number of FITC conjugated lIa stained cells/mm2 at 0, 2 & 18 hours after chemical application. We found that restraint significantly decreased DNFB-induced cellular proliferation in lymph nodes by 50%. We also determined that LC from restrained mice appeared to be rounder with fewer dendritic processes than observed in non restrained mice. In addition, DNFB caused a 50% reduction of the number of LC in the epidermis of non-restrained mice at 18 h, bur only a 39% reduction in LC for restrained mice. These data suggest that restraint-induced decrease in T cell proliferation may be due, in part, to decreased LC migration to the local lymph nodes.
Keywords
Lymph-nodes; Stress; Cell-migration; Sensitization; In-vivo-studies; Laboratory-animals; Animals; Animal-studies
Publication Date
20010301
Document Type
Abstract
Fiscal Year
2001
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
1096-6080
NIOSH Division
HELD
Source Name
The Toxicologist. Society of Toxicology 40th Annual Meeting, March 25-29, 2001, San Francisco, California
State
WV
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