A technique for the identification and direct analysis of hexahydro-1,3,5-tris(2-hydroxyethyl)-s-triazine in metalworking fluids using electrospray-mass spectrometry.
Pretty-J; Glaser-R; Jones-J III; Lunsford-RA
Analyst 2004 Nov; 129(11):1150-1155
Hexahydro-1,3,5-tris(2-hydroxyethyl)-s-triazine is a widely used biocide in metalworking fluids that resists direct quantification in many analytical methods due to instability. It can be detected in electrospray-mass spectrometry (ES-MS) due to the formation of a charged and relatively stable adduct with the sodium ion. This adduct produces a distinct ion spectrum via collision-induced fragmentation, which should promote specific detection of the analyte in complex matrices. ES-MS detection of the analyte added to, or already present in, metalworking fluid samples at microg mL(-1) levels is demonstrated. Parameters affecting the formation and detection of the sodium adduct, including choice of solvent, alkalinity, and sodium ion level are explored. Linearity of response in flow injection mode is demonstrated.
Metalworking-industry; Metalworking-fluids; Metalworking; Mass-spectrometry; Biocides; Analytical-methods; Sodium-compounds; Sampling; Solvents
U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, 4676 Columbia Parkway, Cincinnati, OH 452
Research Tools and Approaches: Exposure Assessment Methods