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Characterization of the in vitro unscheduled DNA synthesis assay in primary lung cells of the rat.

Authors
Whong-WZ; Stewart-JD; Ong-T
Source
Mutat Res 1991 Jan; 262(1):51-55
NIOSHTIC No.
20025497
Abstract
The in vitro unscheduled DNA synthesis (UDS) assay has been evaluated in rat primary lung cells with known genotoxicants. The autoradiographic method was employed to detect UDS in both alveolar macrophages and primary pulmonary cells. Data of a time course study revealed that a high radioactive labeling of DNA repair was achieved after a 16-h incubation with [3H]thymidine. Coupled with low serum (1%), hydroxyurea at the concentration of 20 mM inhibited regular DNA synthesis in primary lung cells in a satisfactory manner (81-88% inhibition). With this protocol, a dose-related increase in UDS was induced by N-methyl-N'-nitro-N-nitrosoguanidine and 2-aminoanthracene in both rat alveolar macrophages and primary lung cells. The results suggest that primary rat lung cells in culture possess DNA-repair ability and that the UDS assay may be useful for assessing the pulmonary genotoxic effect of chemicals in this cell system.
Keywords
In-vitro-studies; Lung-cells; Laboratory-animals; Animals; Animal-studies; Genotoxic-effects; Pulmonary-system; Cell-function; Cell-transformation; Cellular-function; Author Keywords: DNA repair; Primary lung cells; Alveolar macrophages; (Rat); Unscheduled DNA synthesis (UDS)
Contact
Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505
CODEN
MUREAV
Publication Date
19910101
Document Type
Journal Article
Fiscal Year
1991
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
0027-5107
NIOSH Division
DRDS
Source Name
Mutation Research
State
WV
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