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Contact hypersensitivity to dicyclohexylcarbodiimide and diisopropylcarbodiimide in female B6C3F1 mice.

Authors
Hayes-BB; Gerber-PC; Griffey-SS; Meade-BJ
Source
Drug Chem Toxicol 1998 May; 21(2):195-206
NIOSHTIC No.
20025314
Abstract
Dicyclohexylcarbodiimide (DCC) and diisopropylcarbodiimide (DIC) are two commonly used coupling reagents in protein synthesis resulting in exposure of individuals in chemical and pharmaceutical industries as well as research laboratories involved in protein synthesis and recombinant DNA techniques. The objectives of these studies were to determine the irritation and sensitizing potential of these two compounds when applied topically to B6C3F1 mice. Sensitization potential was assessed by the Mouse Ear Swelling Test (MEST) and the murine Local Lymph Node Assay (LLNA). Concentrations used in the contact hypersensitivity assays were determined by primary irritancy studies. DCC and DIC were identified as both irritants and contact sensitizers with the MEST being a more sensitive indicator of sensitization potential. The MEST identified DCC as a sensitizer at concentrations as low as 0.006% (w/v) 24 hr and 48 hr post challenge and DIC at 0.3% (w/v) and 1.5% (w/v) 24 and 48 hr post challenge, respectively. In the LLNA, the lowest concentrations yielding a significant response were 0.06% (w/v) for DCC and 10% (w/v) for DIC.
Keywords
Protein-synthesis; Protein-chemistry; Proteins; Deoxyribonucleic-acids; Irritants; Animal-studies; Animals; Laboratory-animals; Pharmaceutical-industry; Skin-exposure; Skin-irritants
CODEN
DCTODJ
Publication Date
19980501
Document Type
Journal Article
Fiscal Year
1998
NTIS Accession No.
NTIS Price
Issue of Publication
2
ISSN
0148-0545
NIOSH Division
HELD
Source Name
Drug and Chemical Toxicology
State
WV
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