Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Concentration- and time-dependent upregulation and release of the cytokines MIP-2, KC, TNF, and MIP-1alpha in rat alveolar macrophages by fungal spores implicated in airway inflammation.

Authors
Shahan-TA; Sorenson-WG; Paulauskis-JD; Morey-R; Lewis-DM
Source
Am J Respir Cell Mol Biol 1998 Mar; 18(3):435-440
NIOSHTIC No.
20025293
Abstract
Inhalation of fungal spores has been shown to cause primary or secondary infection and respiratory inflammation and diseases such as allergic alveolitis, atopic asthma, and organic dust toxic syndrome, which are rarely reported in the absence of predisposing factors. Biochemical and molecular markers of inflammation were measured in rat bronchial alveolar lavage cells (> 95% macrophages) following stimulation with fungal spores isolated from pathogenic and nonpathogenic fungi that have been implicated in airway inflammation. The results of this study demonstrate that mRNA transcripts for the C-X-C branch of the PF4 superfamily are differentially upregulated over those of the C-C mediators in a time- and concentration-dependent manner. Macrophage inflammatory protein (MIP)-2 and KC were differentially upregulated over the acute phase inflammatory cytokines MIP-1alpha and tumor necrosis factor-alpha (TNF-alpha) in rat alveolar macrophages stimulated with fungal spores from Aspergillus candidus, Aspergillus niger, Eurotium amstelodami, and Cladosporium cladosporioides. Spores from Aspergillus terreus and Penicillium spinulosum failed to stimulate an increase of any cytokine mRNA, whereas those from Aspergillus fumigatus stimulated the upregulation of MIP-2, KC, TNF-alpha, and MIP-1alpha mRNAs. Over time, A. fumigatus stimulated increasing KC production until 24 h, when production levels increased slightly, then leveled off when measurements ceased at 36 h. Latex spheres stimulated modest amounts of MIP-2 and transforming growth factor-beta only. These observations suggest that the inflammatory cytokines MIP-2 and KC may be involved in the inflammation arising from the inhalation of fungal spores in a time- and concentration-dependent manner.
Keywords
Laboratory-animals; Animals; Animal-studies; Fungal-infections; Airway-obstruction; Respiratory-system-disorders; Allergic-disorders; Bronchial-asthma; Organic-dusts; Alveolar-cells; Microorganisms; Aerosols
Contact
W.G. Sorenson, 1095 Willowdale Road, MS-215, CDC/DRDS/NIOSH, Morgantown, WV 26505
CODEN
AJRBEL
Publication Date
19980301
Document Type
Journal Article
Email Address
wgs1@cdc.gov
Fiscal Year
1998
NTIS Accession No.
NTIS Price
Issue of Publication
3
ISSN
1044-1549
NIOSH Division
DRDS
Source Name
American Journal of Respiratory Cell and Molecular Biology
State
WV; MA
TOP