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Detection of genomic instability in lung cancer tissues by random amplified polymorphic DNA analysis.

Authors
Ong-T; Song-B; Qian-H; Wu-ZL; Whong-W
Source
Carcinogenesis 1998 Jan; 19(1):233-235
NIOSHTIC No.
20024942
Abstract
Genomic instability resulting in multiple mutations is believed to be a driving force in the carcinogenic process. In this study, the random amplified polymorphic DNA (RAPD) technique, a simple PCR-based DNA polymorphism assay system, was used for detecting genomic instability in lung cancer tissues. DNAs from 20 lung cancer (18 non-small cell lung cancers and two small cell lung cancers) and their corresponding normal tissues were amplified individually by RAPD with seven different 10-base arbitrary primers. PCR products from RAPD were electrophoretically separated in agarose gels and banding profiles were visualized by ethidium bromide staining. The ability to detect genomic instability in 20 cancer tissues by each single primer ranged from 15 to 75%. DNA changes were detected by at least one primer in 19 (95%) cancer tissues. These results seem to indicate that genomic rearrangement is associated with lung carcinogenesis and that RAPD analysis is useful for the detection of genomic instability in lung cancer tissues.
Keywords
Genes; Carcinogens; Carcinogenesis; Carcinogenicity; Lung-cancer; Lung; Lung-cells; Lung-disease; Lung-disorders; Pulmonary-system-disorders; Respiratory-system-disorders
Contact
Toxicology and Molecular Biology Branch, Health Effects Laboratory Division. National Institute for Occupational Safety and Health. Morgantown WV 26505, USA
CODEN
CRNGDP
Publication Date
19980101
Document Type
Journal Article
Email Address
too2@cdc.gov
Fiscal Year
1998
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
0143-3334
NIOSH Division
HELD
Source Name
Carcinogenesis
State
WV
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