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Guinea pig ozone-induced airway hyperreactivity is associated with increased N-acetyl-beta-D-glucosaminidase activity in bronchoalveolar lavage fluid.

Authors
Lew-DB; Chodimella-V; Murlas-CG
Source
Lung 1990 Sep-Oct; 168(5):273-283
NIOSHTIC No.
20024781
Abstract
High level ozone exposure is known to cause acute, neutrophil-independent airway hyperreactivity in the guinea pig. The precise biochemical mechanisms involved remain unclear. Because of its potential pathophysiologic importance, we examined whether a lysosomal hydrolase, N-acetyl-beta-D-glucosaminidase (NAGA) was released from the airways in vivo and from bronchoalveolar cells, specifically macrophages. Muscarinic reactivity was determined by measuring specific airway resistance (sRaw) in response to increasing doses of aerosolized acetylcholine in guinea pigs that were either exposed to air or to ozone (3.0 ppm, 2 h). The ozone-exposed animals showed substantial muscarinic hyperreactivity 30 min after exposure. In addition, both total and percent released NAGA in bronchoalveolar lavage fluid obtained immediately after reactivity testing were significantly greater in the ozone-exposed group. It was also found that substantially more NAGA was released from mixed bronchoalveolar lavage cells in response to 20 microM A23187. Moreover, bronchoalveolar macrophages of ozone-exposed animals secreted more NAGA upon stimulation in vitro by either 20 microM A23187 or 200 micrograms/ml opsonized zymosan. We conclude that ozone-induced airway hyperreactivity in guinea pigs is associated with the presence of increased NAGA activity in bronchoalveolar fluid. Our data suggest that bronchoalveolar macrophages may, at least in part, be responsible for release of this enzyme into the airways after ozone exposure.
Keywords
Pulmonary-system-disorders; Enzyme-activity; Respiratory-irritants; Lung-irritants; In-vivo-study; Cellular-reactions; Laboratory-animals; Animal-studies; Animals
Contact
Department of Pediatrics, University of Tennessee, Memphis
CODEN
LUNGD9
CAS No.
10028-15-6
Publication Date
19900901
Document Type
Journal Article
Funding Amount
127961
Funding Type
Grant
Fiscal Year
1990
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-K01-OH-00060
Issue of Publication
5
ISSN
0341-2040
Priority Area
Pulmonary-system-disorders
Source Name
Lung
State
OH
Performing Organization
University of Cincinnati, Cincinnati, Ohio
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