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Inhibition of AP-1 and neoplastic transformation by fresh apple peel extract.

Authors
Ding-M; Lu-Y; Bowman-L; Huang-C; Leonard-S; Wang-L; Vallyathan-V; Castranova-V; Shi-X
Source
J Biol Chem 2004 Mar; 279(11):10670-10676
NIOSHTIC No.
20024738
Abstract
Consumption of fruits and vegetables has been associated with a low incidence of cancers and other chronic diseases. Previous studies suggested that fresh apples inhibit tumor cell proliferation. Here we report that oral administration of apple peel extracts decreased the number of nonmalignant and malignant skin tumors per mouse induced by 12-O-tetradecanolyphorbol-13-acetate (TPA) in 7,12-dimethylbenz(a)anthracene-initiated mouse skin. ESR analysis indicated that apple extract strongly scavenged hydroxyl (OH) and superoxide (O(2)(-)) radicals. Mechanistic studies showed that pretreatment with apple peel extract inhibited AP-1 transactivation induced by ultraviolet B irradiation or TPA in JB6 cells and AP-1-luciferase reporter transgenic mice. This inhibitory effect appears to be mediated by the inhibition of ERKs and JNK activity. The results provide the first evidence that an extract from fresh apple peel extract may inhibit tumor promoter-induced carcinogenesis and associated cell signaling, and suggest that the chemopreventive effects of fresh apple may be through its antioxidant properties by blocking reactive oxygen species-mediated AP-1-MAPK activation.
Keywords
Chronic-degenerative-diseases; Chronic-exposure; Cancer; Skin-tumors; Skin-diseases; Skin-cancer; Antioxidants; Antioxidation; Laboratory-animals; Animals; Animal-studies
CODEN
JBCHA3
Publication Date
20040312
Document Type
Journal Article
Email Address
mid5@cdc.gov
Fiscal Year
2004
NTIS Accession No.
NTIS Price
Issue of Publication
11
ISSN
0021-9258
NIOSH Division
HELD
Priority Area
Research Tools and Approaches: Cancer Research Methods
Source Name
Journal of Biological Chemistry
State
WV; NY
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