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Molecular characterization of a voltage-gated potassium channel expressed in rat testis.

Authors
Jacob-A; Hurley-IR; Goodwin-LO; Cooper-GW; Benoff-S
Source
Mol Hum Reprod 2000 Apr; 6(4):303-313
NIOSHTIC No.
20024662
Abstract
Potassium (K(+)) channels are present in both mammalian testis and spermatozoa. Immunofluorescent detection of sperm-bound biotinylated charybdotoxin, an inhibitor of Ca(2+)-activated and of delayed rectifier K(+) channels, indicated that these ion channels are uniformly distributed over the surface of both heads and tails of unfixed rat epididymal spermatozoa. Reverse transcription-polymerase chain reaction (RT-PCR) analysis on rat testis RNA with PCR primers, based on known nucleotide sequences of different classes of K(+) channels, amplified sequences homologous to delayed rectifier K(+) channels. In-situ RT-PCR on rat testis sections showed that these K(+) channel transcripts are present in the cytoplasm of primary spermatocytes and post-meiotic elongating spermatids. Northern blot analysis of various rat tissues identified multiple K(+) channel transcripts, some of which were observed only in testis. An attempt to obtain a full length rat testis K(+) channel cDNA sequence gave an assembled sequence of 2693 base pairs with >90% homology to a delayed rectifier K(+) channel, Kv1.3. A method for rapid amplification of cDNA ends was employed to amplify the 5' sequences of the rat testis cDNA but a unique sequence could not be obtained. DNA sequencer traces suggest that multiple related K(+) channels which differed at their 5' ends were amplified in rat testis.
Keywords
Fertility; Morphology; Biomarkers; Reproductive-hazards; Reproductive-system; Potassium-compounds; Laboratory-animals; Animal-studies; Animals; Spermatozoa; Spermatogenesis
Contact
North Shore University Hospital, 300 Community Drive, Boas-Marks Biomedical Science Research Center - Room 119, Manhasset, New York 11030, USA
CODEN
MHREFD
CAS No.
7440-09-7
Publication Date
20000401
Document Type
Journal Article
Funding Amount
79440
Funding Type
Grant
Fiscal Year
2000
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R03-OH-003584
Issue of Publication
4
ISSN
1360-9947
Priority Area
Research Tools and Approaches: Exposure Assessment Methods
Source Name
Molecular Human Reproduction
State
NY
Performing Organization
North Shore University Hospital, Manhasset, New York
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