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Vanadium-induced alterations in macrophage responses to biological response modifiers in vitro.

Authors
Cohen-MD; Schlesinger-RB; Zelikoff-LT
Source
Toxicologist 1993 Mar; 13(1):422
NIOSHTIC No.
20024495
Abstract
Previous in vivo studies have demonstrated that vanadium impairs host resistance overall, and the antimicrobial activity and function of several intracellular enzymes of macrophages (M+). To determine if the observed immunomodulation might be a result of altered interactions of M, with biological response modifiers, the release/activity of two major cytokines and the production of reactive oxygen intermediates after stimulation of vanadium-treated cultured mouse WEHI-3 M, with lipopolysaccharide (LPS) or interferon-y (IFNy) were assessed. In addition, the binding affinity and numbers of lFNy surface receptors were quantitated. Results demonstrate that the levels and activities of LPS-induced interleukin-l and tumor necrosis factor-a were decreased as vanadium concentrations increased. After treatment of cells with IFNy for 48 hr, hydrogen peroxide (H202) formation in metal-free M, increased - 40% while vanadium-treated cells had decreased H202 formation; similar effects upon superoxide anion formation were also observed. Binding studies indicated that the vanadium-treated M+ had - 500/0 fewer IFNy surface receptors, but the binding affinity of the remaining receptors were 100-400 times greater than those on control M+. This study shows that in vitro exposure to vanadium can alter M-mediated immune responses by modifying their interactions with bioactivating agents. This inability to bind and respond to these external signals could contribute to the overal1 diminution of host immunocompetence observed in vivo.
Keywords
Vanadium-compounds; Biological-factors; In-vitro-studies; In-vivo-studies; Bioactivation
CAS No.
7440-62-2; 7722-84-1
Publication Date
19930301
Document Type
Abstract
Funding Amount
78780
Funding Type
Grant
Fiscal Year
1993
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R03-OH-003064
Issue of Publication
1
ISSN
0731-9193
Priority Area
Other Occupational Concerns
Source Name
The Toxicologist. Society of Toxicology 32nd Annual Meeting, March 14-18,1993, New Orleans, Louisiana
State
NY
Performing Organization
New York University, New York, New York
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