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Two-dimensional electrophoretic protein profile analysis following exposure of human uroepithelial cells to occupational bladder carcinogens.

Authors
Kanitz-MH; Swaminathan-S; Savage-RE Jr.
Source
Cancer Lett 2004 Mar; 205(2):121-131
NIOSHTIC No.
20024438
Abstract
Protein biomarkers to occupational carcinogens were investigated using a transformable human uroepithelial cell system, SV-HUC.PC. SV-HUC.PC was treated with N-hydroxy-4,4'-methylene bis (2-chloroaniline) (N-OH-MOCA) or N-hydroxy-4 aminobiphenyl (N-OH-ABP). Two-dimensional gel electrophoresis of cell lysates compared protein changes across treatments. Increasing N-OH-MOCA resulted in a dose-related increase in protein spots altered. Comparing cell profiles treated with either carcinogen revealed alterations in the expression of nine proteins, identified using the TagIdent database. These demonstrated isoelectric point shift (1) or quantity change (8). Our investigation may be useful in identifying biomarkers of effects of exposure to bladder carcinogens.
Keywords
Bladder-disorders; Carcinogens; Carcinogenesis; Biomarkers; Proteins; Occupational-health; Urogenital-system-disorders
Contact
Division of Applied Research and Technology, Taft Laboratory, National Institute for Occupational Safety and Health, MS/C-23, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
CODEN
CALEDQ
CAS No.
95-51-2; 92-67-1
Publication Date
20040318
Document Type
Journal Article
Email Address
mhk2@cdc.gov
Fiscal Year
2004
NTIS Accession No.
NTIS Price
Issue of Publication
2
ISSN
0304-3835
NIOSH Division
DART
Priority Area
Research Tools and Approaches: Exposure Assessment Methods
Source Name
Cancer Letters
State
OH; WI
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