Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Concurrent fluorescence in situ hybridization and immunocytochemistry for the detection of chromosome aberrations in exfoliated bronchial epithelial cells.

Authors
Neft-RE; Murphy-MM; Teirney-LA; Belinsky-SA; Anderson-M; Saccomanno-G; Michels-R; Timm-S; Gilliland-FD; Crowell-RE; Lechner-JF
Source
Acta Cytol 1997 Nov-Dec; 41(6):1769-1773
NIOSHTIC No.
20024398
Abstract
A procedure was developed to allow concurrent detection of chromosome aberrations and identification of bronchial epithelial cells. Fluorescence in situ hybridization for chromosome 7 and immunocytochemistry for cytokeratin were performed on exfoliated bronchial epithelial cells in a sputum sample from a cancer patient. The Spectrum Orange-labeled alpha satellite probe for chromosome 7 produced red fluorescence, nuclei were counterstained with 4,6-diamidino-2-phenylindole (blue), and cytokeratin was visualized using a fluorescein isothiocyanate (FITC)-conjugated secondary antibody (green). This procedure allowed the rapid identification of airway epithelial cells with numerical chromosome aberrations in this sample. Ultimately, this procedure could increase the sensitivity and specificity of sputum cytology as a laboratory diagnostic tool for the early detection of lung cancer.
Keywords
Risk-factors; Lung-disease; Lung-cancer; Lung-disorders; Pulmonary-system-disorders; Respiratory-system-disorders; Neoplasms; Immunochemistry; Cytochemistry; Bronchial-cancer
Contact
Robin E. Neft, Ph.D., Lovelace Respiratory Research Institute, P.O. Box 5890, Alburquerque, NM 87185
CODEN
ACYTAN
Publication Date
19971101
Document Type
Journal Article
Funding Amount
54000
Funding Type
Grant
Fiscal Year
1998
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-K01-OH-000142
Issue of Publication
6
ISSN
0001-5547
Source Name
Acta Cytologica
State
NM
Performing Organization
University of New Mexico, Department of Medicine, Albuquerque, New Mexico
TOP