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Determination of zinc dialkyldithiocarbamates in latex condoms.

Authors
Depree-GJ; Bledsoe-TA; Siegel-PD
Source
J Chromatogr Sci 2004 Feb; 42(2):80-84
NIOSHTIC No.
20024388
Abstract
A simple high-performance liquid chromatographic (HPLC) assay is developed for measuring zinc dialkyldithiocarbamate (DTC) levels in latex condoms. After extraction of 14 different brands of latex condoms in acetonitrile, aliquots of the extracts are subjected to a preliminary screening assay by treatment with cobalt chloride and measurement of UV absorption at 320 nm, which results in the identification of 6 DTC-containing samples. Prior to analysis by HPLC, zinc dimethyldithiocarbamate (ZDMC) or zinc diethyldithiocarbamate (ZDEC) is added to the extracts in order to block transmetalation reactions with the analytes of interest. A reversed-phase C18 column, with gradient elution and UV detection at 260 nm, is used to measure the zinc DTCs. The limits of detection for ZDEC and zinc dibutyldithiocarbamate (ZDBC) are 5 and 10 micrpg/mL. Levels of ZDBC and ZDEC range from not detectable to 3.31 and 1.79 mg/condom, respectively. Total protein and latex allergenic protein levels are determined and range from 98 to 776 and 0.01 to 14.04 microg/unit, respectively, but are not related to the level of ZDBC or ZDEC. This methodology provides both screening and specific tools for the determination of unstable zinc DTC complexes in latex products.
Keywords
Zinc-compounds; Chromatographic-analysis; Acetones; Nitriles; Cobalt-compounds; Chlorides
Contact
Paul D. Siegel, Ph.D., Analytical Services Branch, M/S L4218, NIOSH, 1095 Willowdale Road, Morgantown, WV 26505-2888
CODEN
JCHSBZ
CAS No.
7440-66-6; 75-05-8
Publication Date
20040201
Document Type
Journal Article
Email Address
pds3@cdc.gov
Fiscal Year
2004
NTIS Accession No.
NTIS Price
Issue of Publication
2
ISSN
0021-9665
NIOSH Division
HELD
Priority Area
Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
Source Name
Journal of Chromatographic Science
State
WV
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