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Quantitative analysis of particle translocation within the lung.

Authors
Fering-J; Soderholm-S; Oberdorster-G
Source
Am Rev Respir Dis 1992 Apr; 145(4)(Pt 2)(Meeting Abstracts):A801
NIOSHTIC No.
20024382
Abstract
We have shown that ultrafine particles (diameter -20 nm) elicit an acute inflammatory response, among others, after being deposited in the alveoli. The inflammatory response seems to be a sequelae of the interstitialization process which is substantially increased in the case of the ultrafine particles compared to particles of larger size(1). The translocation of deposited particles from the alveoli into the pulmonary tissue per se is of interest in particle-lung interaction studies. We have now developed two approaches for quantitative determination of particles in various rat lung compartments during and as a result of translocation. One is based on an extensive lavage of the excised lung, followed by chemical analysis of particle content in the lavaged lung and extra-pulmonary lymph nodes, and of the lavaged cells and fluid. The latter is assumed to be indicative of particles localized in the alveoli, the former of translocated particles. The second approach is based on magnetometry. Using an array of eight flux-gate magnetometers the particle lung burden of magnetite particles is analyzed in vivo. This noninvasive measurement is independent of the location of the magnetic material within the lung, a significant improvement over previous magnetometric systems. After magnetization of the particles by an external magnetic field, the magnetic signal and its relaxation over 15 min. are recorded. This measurement makes it possible to assess the particle lung burden over long periods of time. The use of signal relaxation has the potential to distinguish between intra- and extracellular material in vivo. The present interpretation of the results indicate that after instillation or inhalation of -1 mg of magnetite, the clearance halftime of "relaxing" material is 40-80 days, as expected from other studies. These results seem to be consistent with approaches using chemistry for quantitative analysis. The magnetometric method is less sensitive than chemical analysis and is limited to particles with magnetic properties like magnetite, but it has the potential for repeated noninvasive measurements of distinct particle populations in the same animal.
Keywords
Quantitative-analysis; Pulmonary-system-disorders; Respiratory-system-disorders; Lung-function; Laboratory-animals; Animal-studies; Animals; Lung-burden; In-vivo-studies; Nanotechnology
CODEN
ARDSBL
Publication Date
19920401
Document Type
Abstract
Funding Amount
832522
Funding Type
Grant
Fiscal Year
1992
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-002772
Issue of Publication
4
ISSN
0003-0805
NIOSH Division
HELD
Priority Area
Pulmonary System Disorders
Source Name
American Review of Respiratory Disease
State
NY; WV
Performing Organization
University of Rochester, Rochester, New York
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