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Determination of 3-amino-5-mercapto-1,2,4-triazole in serum.

Authors
Depree-GJ; Siegel-PD
Source
J Chromatogr B 2004 Mar; 801(2):359-362
NIOSHTIC No.
20024352
Abstract
A high performance liquid chromatography (HPLC) method using fluorescence detection to determine 3-amino-5-mercapto-1,2,4-triazole (AMT) levels in serum has been developed. Sample preparation involved treatment with tributylphosphine (TBP) to reduce disulfides formed during storage, precipitation of proteins with acetonitrile (ACN), and precolumn derivatization using the thiol reactive fluorescent probe monobromobimane (MBB). The conjugate (AMT-MBB) was resolved by gradient elution from a C(18) reversed-phase column. The assay method was linear over a concentration range of 0.78-50 microg/ml and had a limit of detection (LOD) of 0.05 microg/ml AMT (10 microl injection). This method provides a sensitive and specific tool for the determination of AMT in serum and may have potential industrial hygiene application.
Keywords
Industrial-hygiene; Sampling; Sampling-methods; Proteins; Antioxidants; Occupational-exposure; Environmental-exposure; Analytical-methods; Analytical-chemistry
Contact
Analytical Services Branch, M/S L4218, 1095 Willowdale Road, Morgantown, WV 26505-2888, USA
CODEN
JCBAAI
CAS No.
998-40-3; 75-05-8
Publication Date
20040305
Document Type
Journal Article
Email Address
pds3@cdc.gov
Fiscal Year
2004
NTIS Accession No.
NTIS Price
Issue of Publication
2
ISSN
1570-0232
NIOSH Division
HELD
Priority Area
Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
Source Name
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
State
WV
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