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Procedure for the quantification of the biomarker (2-methoxyethoxy)acetic acid in human urine samples.

Authors
B'Hymer-C; Cheever-KL; Butler-MA; Brown-KK
Source
J Chromatogr B 2003 Sep; 795(1):145-150
NIOSHTIC No.
20023604
Abstract
An accurate and precise procedure was developed for the detection and quantification of (2-methoxyethoxy)acetic acid (MEAA), a metabolite and biomarker for human exposure to 2-(2-methoxyethoxy)ethanol. The compound 2-(2-methoxyethoxy)ethanol has a wide array of industrial applications including its use as an additive in military jet fuel. Exposure to 2-(2-methoxyethoxy)ethanol is a health concern owing to its toxicity which includes developmental and teratogenic properties. Sample preparation consisted of liquid-liquid extraction (LLE) and esterification of MEAA to produce the ethyl ester. Measurement was by a gas chromatograph (GC) equipped with a mass selective detector (MSD) using a HP-1 capillary column. Recovery studies of spiked blank urine demonstrated good accuracy and precision; recovery varied between 95 and 103% with relative standard deviations of 8.6% and less. The limit of detection (LOD) for this procedure was found to range from 0.02 to 0.08 ug/ml equivalent levels of MEAA in urine. These data and other aspects of the validation of this procedure will be discussed.
Keywords
Acetic-acids; Ethanols; Metabolites; Gas-chromatography; Jet-engine-fuels; Developmental-disorders; Teratogenesis; Esters; Gas-detectors; Urinalysis
Contact
National Institute for Occupational Safety and Health, Division of Applied Research and Technology, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
CODEN
JCBAAI
CAS No.
111-77-3; 16024-56-9
Publication Date
20030925
Document Type
Journal Article
Email Address
cbhymer@cdc.gov
Fiscal Year
2003
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
1570-0232
NIOSH Division
DART
Priority Area
Research Tools and Approaches: Exposure Assessment Methods
Source Name
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
State
OH
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