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Surface sampling methods for Bacillus anthracis spore contamination.

Authors
Sanderson-WT; Hein-MJ; Taylor-L; Curwin-BD; Kinnes-GM; Seitz-TA; Popovic-T; Holmes-HT; Kellum-ME; McAllister-SK; Whaley-DN; Turpin-EA; Walker-T; Freed-JA; Small-DS; Klusartiz-B; Bridges-JH
Source
Emerg Infect Dis 2002 Oct; 8(10):1145-1151
NIOSHTIC No.
20023530
Abstract
During an investigation conducted December 17-20, 2001, we collected environmental samples from a U.S. postal facility in Washington, D.C., known to be extensively contaminated with Bacillus anthracis spores. Because methods for collecting and analyzing B. anthracis spores have not yet been validated, our objective was to compare the relative effectiveness of sampling methods used for collecting spores from contaminated surfaces. Comparison of wipe, wet and dry swab, and HEPA vacuum sock samples on nonporous surfaces indicated good agreement between results with HEPA vacuum and wipe samples. However, results from HEPA vacuum sock and wipe samples agreed poorly with the swab samples. Dry swabs failed to detect spores >75% of the time when they were detected by wipe and HEPA vacuum samples. Wipe samples collected after HEPA vacuum samples and HEPA vacuum samples collected after wipe samples indicated that neither method completely removed spores from the sampled surfaces.
Keywords
Surface-properties; Vacuum-equipment; Postal-employees; Sampling-equipment; Sampling; Sampling-methods; Environmental-contamination; Biohazards; Biological-warfare-agents; Biological-weapons; Biological-agents; Environmental-hazards; Microorganisms
CODEN
EIDIFA
Publication Date
20021001
Document Type
Journal Article
Email Address
wayne-sanderson@uiowa.edu
Fiscal Year
2003
NTIS Accession No.
NTIS Price
Issue of Publication
10
ISSN
1080-6040
NIOSH Division
DSHEFS
Source Name
Emerging Infectious Diseases
State
GA; DC; OH
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