Synthesis characterization and use of 2-[(2H9)butoxy]acetic acid and 2-(3-methylbutoxy)acetic acid as an internal standard and an instrument performance surrogate respectively for the gas chromatographic-mass spectrometric determination of 2-butoxyacetic acid a human metabolite of 2-butoxyethanol.
Brown-KK; Cheever-KL; Butler-MA; Shaw-PB; McLaurin-JL
J Chromatogr B 2003 Jul; 792(2):153-166
2-[(2H9)Butoxy]acetic acid and 2-(3-methylbutoxy)acetic acid were synthesized, mixed with 2-butoxyacetic acid, and separated by capillary gas chromatography on a fused-silica column with a length of 50 m, inside diameter of 0.200 mm, and a "free fatty acid phase" wall coating of 0.3 um film. 2-[(2H9)Butoxy]acetic acid, 2-butoxyacetic acid, and 2-(3-methylbutoxy)acetic acid were baseline resolved at retention times of 13.55, 13.78, and 15.20 min; 2-(3-methylbutoxy)acetic acid having a peak efficiency of 360000. Mass spectrometric detection using selected ion monitoring at m/z 66, 57, and 71 showed linear analytical responses from 0.04 ng to at least 200 ng with a limit of detection of 0.04 ng for 2-butoxyacetic acid.
Chemical-synthesis; Chemical-reactions; Chemical-analysis; Metabolites; Acids; Chromatographic-analysis; Gas-chromatography; Mass-spectrometry; Acetic-acids
National Institute for Occupational Safety and Health (NIOSH), 4676 Columbia Parkway, R-7, Cincinnati, OH 45226, USA
Research Tools and Approaches: Exposure Assessment Methods
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences