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Two assays for urinary N-acetyl-Beta-D glucosaminidase compared.

Authors
Drake-P; Krieg-E; Teass-A; Vallyathan-V
Source
Clin Chem 2002 Sep; 48(9):1604-1605
NIOSHTIC No.
20023481
Abstract
In the course of investigating the exposure of gold miners and other workers to mercury, investigators at the National Institute for Occupational Safety & Health (NIOSH) measured urine N-acetyl--D-glucosaminidase (NAG; EC 3.2.1.52) activity to monitor for renal injury. Numerous methods are available for the urinalysis of NAG activity. Most of the analyses for the NIOSH studies were performed by Pacific Toxicology Laboratories, using the Kamiya Biomedical Company reagent set, which was based on NAG-catalyzed hydrolysis of 6-methyl-2-pyridyl-N-acetyl-1-thio-beta-D-glucosaminide to 6-methyl-2-pyridinethiol. However, while planning our most recent investigational visit to a gold mine, we learned that the Kamiya Biomedical reagent set was temporarily out of stock. Consequently, we decided to perform the analyses in-house using the reagent set manufactured by Boehringer Mannheim Biochemica, which we had used previously for the determination of NAG in rats. This reagent set is based on NAG-catalyzed hydrolysis of the sodium salt of 3-cresolsulfonphthaleinyl-N-acetyl--D-glucosaminide to give 3-cresol purple (3-cresolsulfonphthalein, sodium salt). Because the two assays are based on the hydrolysis of different substrates, and in light of the differences reported among four NAG assays (including the 3-cresol purple) , we suspected that the 3-cresol purple assay might not give NAG activity results equivalent to those obtained by the 6-methyl-2-pyridinethiol assay used in previous investigations. To obtain data with which to compare the two assays, we applied both assays to 72 urine specimens.
Keywords
Miners; Mining-industry; Mercury-compounds; Renal-absorption; Renal-toxicity; Urinalysis; Humans; Heavy-metals
Contact
National Institute for Occupational Safety and Health, Spokane Research Laboratory, 315 East Montgomery Avenue, Spokane, WA 99207
CODEN
CLCHAU
CAS No.
143-74-8
Publication Date
20020901
Document Type
Journal Article
Email Address
pdrake@cdc.gov
Fiscal Year
2002
NTIS Accession No.
NTIS Price
Issue of Publication
9
ISSN
0009-9147
NIOSH Division
SRL; DART; HELD
Source Name
Clinical Chemistry
State
WA; OH; WV
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