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Identification of organic fractions of diesel exhaust particulate (DEP) which inhibit nitric oxide (NO) production from a murine macrophage cell line.

Authors
Saxena-QB; Saxena-RK; Siegel-PD; Lewis-DM
Source
Toxicol Lett 2003 Aug; 143(3):317-322
NIOSHTIC No.
20023415
Abstract
Diesel exhaust particulates (DEPs) can constitute a large component of the particulate air pollution in urban areas and is a health concern. The effects of DEP on nitric oxide (NO) production by a murine macrophage cell line (RAW264.7) in response to interferon-gamma (INFgamma), lipopolysaccharide, (LPS) and Bacillus Calmette-Guerin (BCG) were studied. The DEP was fractionated into organic and inorganic fractions (carbonaceous core). The organic portion was further divided into asphaltene, saturates, less polar aromatics, more polar aromatics and resins-containing fractions. Each fraction was tested for the ability to suppress NO production from BCG-stimulated macrophages. DEP crude organic extract, more polar aromatic hydrocarbon, and resin fractions dose-dependently inhibited BCG-stimulated NO production. It is concluded that the responsiveness of the macrophages to stimuli, such as BCG, is suppressed by DEP and that this activity is most predominant in the polar aromatic hydrocarbons and resins-containing fractions.
Keywords
Diesel-exhausts; Diesel-emissions; Airborne-particles; Aromatic-hydrocarbons; Nitrogen-compounds; Airborne-particles; Particulates; Analytical-methods; Analytical-chemistry
CODEN
TOLED5
Publication Date
20030828
Document Type
Journal Article
Fiscal Year
2003
NTIS Accession No.
NTIS Price
Issue of Publication
3
ISSN
0378-4274
NIOSH Division
HELD
Source Name
Toxicology Letters
State
WV
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