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The SH4-unique-SH3-SH2 domains dictate specificity in signaling that differentiate c-yes from c-src.

Authors
Summy-JM; Qian-Y; Jiang-BH; Koay-Guappone-A; Gatesman-A; Shi-X; Flynn-DC
Source
J Cell Sci 2003 Mar; 116(12):2585-2597
NIOSHTIC No.
20023198
Abstract
c-Src and c-Yes are highly homologous members of the Src family of non-receptor tyrosine kinases. The overall sequence similarity between c-Src and c-Yes allows them to perform many overlapping functions. However, the phenotypes of the c-src and c-yes knockout mice, and cells derived from them, are quite different, indicating functional specificity between the two proteins. Specifically, c-src-/- cells are deficient in several processes that require dynamic regulation of the actin cytoskeleton. In order to begin to understand why c-Yes is unable to compensate for c-Src signaling, we used a series of Src/Yes chimeras in which the non-catalytic functional domains of Src527F were replaced by those of c-Yes. Using chicken embryo fibroblasts as a model system, our results indicate that the c-Yes N-terminal SH4-Unique domains are sufficient to inhibit the ability of Src527F to alter cell morphology, induce actin filament rearrangements or stimulate motility or invasive potential. The data also indicate that the SH4-Unique-SH3-SH2 domains of c-Yes work cooperatively and prevent activation of signaling proteins associated with Src527F transformation, including activation of phosphatidylinositol 3-kinase, phosphorylation of c-Raf and Akt and downregulation of RhoA-GTP. These data indicate that c-Yes may not modulate signals associated with c-Src-induced changes in actin filament integrity and may explain why c-Yes fails to compensate for c-Src signaling in src-/- cells.
Keywords
Cell-morphology; Cell-biology; Cell-function; In-vitro-study; Proteins; Protein-chemistry
CODEN
JNCSAI
Publication Date
20030307
Document Type
Journal Article
Email Address
dflynn@hsc.wvu.edu
Fiscal Year
2003
NTIS Accession No.
NTIS Price
Issue of Publication
12
ISSN
0021-9533
NIOSH Division
HELD
Source Name
Journal of Cell Science
State
WV; TX
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