Early markers of skeletal muscle injury revealed by cDNA array.
Summan-M; Hulderman-T; McKinstry-M; Warren-GL; Simeonova-PP
FASEB J 2003 Mar; 17(5):A1365
Traumatic skeletal muscle injuries result in profound histopathological changes and loss of muscle function. Much research has focused on trying to understand the cellular and molecular mechanisms of injury demarcation and activation of repair processes unique to the skeletal muscle. Here, a cDNA array was used to examine differential gene expression in a tibialis anterior (TA) muscle injury mouse model in ( order to gain an insight to the early mediators involved in skeletal muscle injury/repair mechanisms. cDNA was prepared from the T A muscle of injured and control mice and labeled with the fluorescent dye Cy5 or Cy3 prior to hybridization to a microarray. The microarray analysis, including 715 genes, was conducted ill triplicate and only genes modulated by the injury showing a differential expression (both increased and decreased) 1.7-fold or greater (p < 0.05) than control uninjured T A muscle are described. The injury resulted in the transcriptional upregulation in 2.8% of the genes, including genes associated with inflammation and chemotaxis (1.9 - 7.9-fold upregulated); genes that encode for growth factors and/or cell cycle regulators (1.8 - 2.5-fold upregulated) and genes that encode for myoblast structural components (1.7 - 4.4-fold upregulated). The expression of 3.2% of the genes was down regulated. These include genes involved in energy production, intermediary metabolism and cell signaling/transcription factors (0.4 - 0.7 -fold down regulated). The expression of selected genes was confirmed by real time RT -PCR and the ribonuclease protection assay (RPA), in both the direction and magnitude of the gene expression observed by the cDNA array. These results provide insight into the initial events of skeletal muscle injury.
Musculoskeletal-system; Muscle-function; Muscles; Muscular-disorders; Musculoskeletal-system-disorders; Histopathology; Injuries; Cellular-function; Animal-studies; Genetics; Genetic-factors; Muscle-cells
Abstract; Conference/Symposia Proceedings
The FASEB Journal, Experimental Biology 2003, San Diego, California, April 11-15, 2003