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Mechanical and bioelectric responses of guinea-pig airways to mucosal hyperosmolar (HO) and isosmolar (IO) solutions.

Authors
Fedan-JS; Wu-DXY; Dowdy-JA; Van Scott-MR
Source
FASEB J 2003 Mar; 17(5):A1046
NIOSHTIC No.
20023068
Abstract
Asthmatics experience obstruction during exercise, due to an increase in the osmolarity of the airway surface liquid, and after inhalation of HO aerosols. The mechanism of the response to changes in osmolarity was examined by comparing the effects of mucosal HO (osmolyte added to Krebs solution) and I0 (osmolyte added to water) solutions on mechanical responses of serosal methacholine (3xIO.7 M)-contracted perfused trachea (PT) and short circuit current (Isc) responses of the epithelium. HO D-mannitol (D-M), NMDG-gluconate (N-G), urea (U), NaCl or KCI caused relaxation of the PT. Perfusion with I0 solutions of these osmolytes caused variable responses (contraction, contraction/relaxation, no response, or, rarely, relaxation), but subsequent HO addition of the same osmolyte to the I0 perfusate always triggered relaxation. HO D-M, N-G and U decreased Isc, whereas HO NaCI and KCI increased Isc. I0 solutions of the three ionic osmolytes, N-G, NaCI and KCI, decreased Isc, but subsequent HO addition of these osmolytes stimulated Isc responses similar to those obtained after adding them to Krebs solution. Thus, the mechanical responses to HO or I0 osmolyte solutions were independent of the ionic nature or permeance of the solute. Relaxation was associated with an increase in osmolarity. The bioelectric effects of I0 solutions of osmolytes were similar, but those of HO solutions were agent-specific.
Keywords
Airway-obstruction; Aerosols; Bioelectric-effects; Mechanical-properties; Respiratory-system-disorders; Pulmonary-system-disorders; Animal-studies; Laboratory-animals; Bronchial-asthma
CODEN
FAJOEC
Publication Date
20030322
Document Type
Abstract; Conference/Symposia Proceedings
Fiscal Year
2003
NTIS Accession No.
NTIS Price
Issue of Publication
5
ISSN
0892-6638
NIOSH Division
HELD
Priority Area
Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
Source Name
The FASEB Journal, Experimental Biology 2003, San Diego, California, April 11-15, 2003
State
WV; NC
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