Effects of paving asphalt fume exposure on genotoxic and mutagenic activities in the lung.
Zhao-H; Yin-X; Frazer-D; Barger-M; Tomblyn-S; Stone-S; Ma-J; Castranova-V; Ma-J
FASEB J 2002 Mar; 16(5):A962
Asphalt fumes are complex mixtures of aerosols and vapors containing various organic compounds, including polycyclic aromatic hydrocarbons, and have been demonstrated to alter the cytochrome P-450 system in the lung with significant induction of CYPIAl, The present study was carried out to characterize the potential mutagenic and genotoxic effects of inhaled asphalt fume in a rat model, Rats were exposed to air or asphalt fume generated at paving temperature (-150°C) at --98 mgim3 and 6h/day for 5 days Alveolar macrophages (AM) were obtained by bronchoalveolar lavage, S9 was isolated from the lung tissue, Mutagenicity was monitored using the Ames test and genotoxicity was determined by Comet assay. In comparison to controls, AM from asphalt fume-exposed rats showed significant DNA damage as demonstrated by the Comet assay. However. the 89 fraction from asphalt exposed rat lungs used for metabolic activation of 2-aminoanthracene or benzo[a]pyrene in Ames tests, did not induce mutagenic activity in either S. typhimurium YGIO24 or YGIO29. These results suggest that short term inhalation of asphalt fume did not induce 89 metabolic activity-dependent mutagenicity, but caused significant DNA damage in AM.
Asphalt-fumes; Aerosols; Vapors; Organic-compounds; Polycyclic-aromatic-hydrocarbons; Lung; Mutagens; Mutagenicity; Genotoxic-effects; Animal-studies; Lung-tissue; Metabolic-activation; Liver-enzymes; In-vitro-study; Fumes; Exposure-levels; Aerosol-particles; Lung-cells; Laboratory-animals; Animals
NIOSH, 1095 Willowdale Road, Morgantown, WV 26505
Abstract; Conference/Symposia Proceedings
Work Environment and Workforce: Mixed Exposures
The FASEB Journal, Experimental Biology 2002, New Orleans, Louisiana, April 20-24, 2002