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Kinetics of changes in lymphocyte sub-populations in mouse lungs after intrapulmonary infection with M. bovis (Bacillus Calmette-Guerin) and identity of cells responsible for IFNgamma responses.

Authors
Saxena-RK; Weissman-D; Saxena-QB; Simpson-J; Lewis-DM
Source
Clin Exp Allergy 2002 Jun; 128(3):405-410
NIOSHTIC No.
20022967
Abstract
Gamma interferon (IFN) plays a key role in host defense against pulmonary mycobacterial infections. A variety of lymphocyte subsets may participate in producing pulmonary IFN responses, but their relative contributions after mycobacterial infection have not been clearly elucidated. To address this question, C57Bl/6 female mice were infected by intrapulmonary instillation of 25 104 BCG (Mycobacterium bovis Bacillus Calmette-Guerin). Lymphocyte populations in lung interstitium were examined at different time points after the infection. BCG load in lungs peaked between 4 and 6 weeks post-infection and declined to very low levels by the 12th week of infection. Recovery of lung interstitial lymphocytes doubled by 4-6 weeks after infection and declined thereafter. Flow cytometric analysis of the lung-derived lymphocytes revealed that about 5% of the these cells made IFN in control mice, and this baseline IFN production involved T (CD3+NK1.1), NK (CD3NK1.1+) and NKT (CD3+NK1.1+) cells. As the BCG lung infection peaked, the total number of CD3+ T cells in the lungs increased threefold at 5-6 weeks post-infection. There was a marked increase (sixfold) in the number of T cells secreting IFN 5-6 weeks post-infection. Some increase was also noted in the NKT cells making IFN, but the numbers of NK cells making IFN in BCG-infected lungs remained unaltered. Our results suggest that whereas NK and NKT cells contribute to baseline IFN secretion in control lungs, expansion in the IFN-producing T-cell population was essentially responsible for the augmented response seen in lungs of BCG-infected mice.
Keywords
Pulmonary-disorders; Pulmonary-function; Lymphocytes; Animal-studies; Laboratory-animals; Cell-cultures; Cell-morphology; Microorganisms
Contact
Dr; Daniel M. Lewis, Analytical Services Branch, HELD, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505, USA
CODEN
CLEAEN
Publication Date
20020101
Document Type
Journal Article
Email Address
dml1@cdc.gov
Fiscal Year
2002
NTIS Accession No.
NTIS Price
Issue of Publication
3
ISSN
0954-7894
NIOSH Division
HELD
Priority Area
Disease and Injury: Infectious Diseases
Source Name
Clinical and Experimental Allergy
State
WV
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