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Asphalt exposure enhances neuropeptide levels in sensory neurons projecting to the rat nasal epithelium.

Authors
Sikora-E; Stone-S; Tomblyn-S; Frazer-D; Castranova-V; Dey-R
Source
J Toxicol Environ Health, A 2003 Jun, 66(11):1015-1027
NIOSHTIC No.
20022961
Abstract
Asphalt fumes have been reported to produce nasal irritation in road workers. Since inhaled irritants can increase substance P (SP) production in airway neurons, the effects of asphalt fumes on SP production in trigeminal ganglia (TG) sensory neurons innervating the nasal mucosa were investigated. The effects of asphalt fumes on nasal mucosal innervation were examined by measuring SP and calcitonin-gene-related peptide (CGRP) levels in rat TG neurons projecting to the nasal epithelium. Female Sprague-Dawley rats were exposed to asphalt fumes at 16.0 +/- 8.1mg /m3 for 5 consecutive days, 3.5 h/d. Inflammatory cells were measured in nasal cavity lavage fluid. SP and CGRP immunoreactivity (IR) was measured in the cell bodies of trigeminal ganglion sensory neurons projecting to the nasal cavity. A significant increase in neutrophils and macrophages was observed after asphalt fume exposure indicating an inflammatory response in the nasal cavity. The percentage of SP-IR neurons increased significantly in the asphalt-exposed rats, and the proportion of CGRP-IR neurons was also elevated following asphalt exposure. These results indicate that exposure to asphalt fumes produces inflammation and increases the levels of SP and CGRP in TG neurons projecting to the nasal epithelium. The findings are consistent with asphalt-induced activation of sensory C-fibers in the nasal cavity. Enhanced sensory neuropeptide release from nerve terminals in the nasal cavity may produce neurogenic inflammation associated with nasal irritation following exposure to asphalt fumes.
Keywords
Asphalt-fumes; Animal-studies; Nasal-cavity; Genes; Peptides; Cell-function; Laboratory-animals; Hydrocarbons; Aromatic-hydrocarbons; Polycyclic-hydrocarbons; Polycyclic-aromatic-hydrocarbons
Contact
Dr. Richard D. Dey, Department of Neurobiology and Anatomy, Robert C. Byrd Heath Sciences Center, PO Box 9128, Morgantown, WV 26506-9128, USA
CODEN
JTEHD6
Publication Date
20030606
Document Type
Journal Article
Email Address
rdey@hcs.wvu.edu
Fiscal Year
2003
NTIS Accession No.
NTIS Price
Issue of Publication
11
ISSN
0098-4108
NIOSH Division
HELD
Source Name
Journal of Toxicology and Environmental Health, Part A: Current Issues
State
WV
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