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Determination of alachlor and its metabolites in rat plasma and urine by liquid chromatography - electrospray ionization mass spectrometry.

Authors
Zang-LY; DeHaven-J; Yocum-A; Qiao-G
Source
J Chromatogr B 2002 Feb; 767(1):93-101
NIOSHTIC No.
20022917
Abstract
A method based on liquid chromatography (LC) in combination with mass spectrometry (MS) for the analysis of alachlor (ALA) and its metabolites, 2-chloro-N-[2,6-diethylphenyl]acetamide (CDEPA) and 2,6-diethylaniline (DEA), in rat plasma and urine has been developed. 13C-labeled ALA was used as the internal standard for quantitation. The analyte in plasma or urine was isolated using a Waters Oasis HLB extraction plate. The mass spectrometer was operated in the ESI MS-SIM mode with a programming procedure. The retention times for ALA, CDEPA and DEA were 1.84, 3.11 and 4.12 min, respectively. The limits of quantification (LOQ) for ALA, CDEPA and DEA were 2.3, 0.8 and 0.8 ng per injection, respectively. The linear fit of analyte to mass response had an R2 of 0.99. Reproducibility of the sample handling and LC-MS analysis had a RSD of < or = 10%. The average recoveries for these analytes in rat plasma were better than 90%. Similar results were obtained with rat urine.
Keywords
Metabolites; Urinalysis; Mass-spectrometry; Ionization; Laboratory-animals; Animal-studies; Animals; Carcinogens; In-vitro-study; Liver; In-vivo-study
Contact
Lun-Yi Zang, Exposure Assessment Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, MS 3030, Morgantown, WV 26505-2888, USA
CODEN
JCBAAI
CAS No.
15972-60-8
Publication Date
20020205
Document Type
Journal Article
Email Address
laz7@cdc.gov
Fiscal Year
2002
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
1570-0232
NIOSH Division
HELD
Source Name
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
State
WV
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