Transcriptomic profiles in rat skin following dermal exposure to sodium lauryl sulfate.
Garrett-CM; Rogers-JV; Wang-C; McDougal-JN
Toxicologist 2002 Mar; 66(1-S):161
Dermal irritation continues to be an important yet poorly understood occupational health issue. Irritant contact dermatitis (ICD), the most common occupational skin disease, costs government and industry millions of dollars each year. Gene array technology can serve as a tool for assessing chemical irritancy potential. To determine the transcriptional response to dermal irritants, male Fisher 344 rat skins were exposed to 10% sodium lauryl sulfate (SLS, a model skin irritant) for 1 h in vivo. Skin total RNA was isolated at 0 h (control), and 1 and 4 hrs following the beginning of the exposure to monitor transciptomic profiles using the Affymetrix RatTox U34 array. Our results indicated that dermal exposure to SLS resulted in a significant change of gene expressions: more than 20 genes (ie., inflammatory, Bcl-2-related, oncogenes) were increased >2-fold, whereas more than 24 genes (ie., cytochrome P450-related, tumor suppressor) showed a >2-fold decrease (1h vs 0 h controls). By 4 hr, more than 60 genes (ie., inflammatory, oxidative and cellular stress, cell cycle, transcription factors, Bcl-2-related) were up-regulated and more than 10 genes (transcription factors, junctional proteins) were down-regulated by a factor >2-fold when compared to the 0 h controls. A strong temporal change of gene expression was observed in the skin following exposure to SLS. Characterization and analysis of the transcriptomic response of skin to SLS can enhance the risk assessment of dermal irritants.
In-vitro-study; Stress; Cytotoxicity; In-vivo-study; Exposure-methods; Skin-exposure; Dermatitis; Dermatosis; Skin-diseases; Skin-disorders; Skin-irritants; Skin-lesions; Laboratory-animals; Animal-studies
Disease and Injury: Allergic and Irritant Dermatitis
The Toxicologist. Society of Toxicology 41st Annual Meeting and ToxExpo, March 17-21, 2002, Nashville, Tennessee
Wright State University, Dayton, Ohio