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Transcriptomic profiles in rat skin following dermal exposure to sodium lauryl sulfate.

Authors
Garrett-CM; Rogers-JV; Wang-C; McDougal-JN
Source
Toxicologist 2002 Mar; 66(1-S):161
NIOSHTIC No.
20022882
Abstract
Dermal irritation continues to be an important yet poorly understood occupational health issue. Irritant contact dermatitis (ICD), the most common occupational skin disease, costs government and industry millions of dollars each year. Gene array technology can serve as a tool for assessing chemical irritancy potential. To determine the transcriptional response to dermal irritants, male Fisher 344 rat skins were exposed to 10% sodium lauryl sulfate (SLS, a model skin irritant) for 1 h in vivo. Skin total RNA was isolated at 0 h (control), and 1 and 4 hrs following the beginning of the exposure to monitor transciptomic profiles using the Affymetrix RatTox U34 array. Our results indicated that dermal exposure to SLS resulted in a significant change of gene expressions: more than 20 genes (ie., inflammatory, Bcl-2-related, oncogenes) were increased >2-fold, whereas more than 24 genes (ie., cytochrome P450-related, tumor suppressor) showed a >2-fold decrease (1h vs 0 h controls). By 4 hr, more than 60 genes (ie., inflammatory, oxidative and cellular stress, cell cycle, transcription factors, Bcl-2-related) were up-regulated and more than 10 genes (transcription factors, junctional proteins) were down-regulated by a factor >2-fold when compared to the 0 h controls. A strong temporal change of gene expression was observed in the skin following exposure to SLS. Characterization and analysis of the transcriptomic response of skin to SLS can enhance the risk assessment of dermal irritants.
Keywords
In-vitro-study; Stress; Cytotoxicity; In-vivo-study; Exposure-methods; Skin-exposure; Dermatitis; Dermatosis; Skin-diseases; Skin-disorders; Skin-irritants; Skin-lesions; Laboratory-animals; Animal-studies
CAS No.
151-21-3
Publication Date
20020301
Document Type
Abstract
Funding Amount
777420
Funding Type
Grant
Fiscal Year
2002
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-003654
ISSN
1096-6080
Priority Area
Disease and Injury: Allergic and Irritant Dermatitis
Source Name
The Toxicologist. Society of Toxicology 41st Annual Meeting and ToxExpo, March 17-21, 2002, Nashville, Tennessee
State
OH
Performing Organization
Wright State University, Dayton, Ohio
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