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Is there a relationship between urinary and leukocyte 8-hydroxydeoxyguanosine in humans?

Toraason-M; Mathias-P; Ruder-A; Butler-MA; Taylor-L; Forrester-C; Krieg-E; DeBord-DG
Toxicologist 2002 Mar; 66(1-S):280
8-Hydoxydeoxyguanosine (8-OHdG) is typically measured in DNA of white blood cells (WBCs) or in urine. Urine measurement is believed to provide an index of oxidative DNA damage repair. WBCs are considered a surrogate for target tissues. These markers are used almost interchangeably to assess the adverse effects of a variety of environmental factors. Only a few of the hundreds of studies assessing 8-OHdG as a biomarker of oxidative DNA damage in humans, have measured 8-OHdG in both urine and WBC DNA. Two recent studies reported non-significant inverse associations between urinary excretion of 8-OHdG and WBC DNA 8-OHdG. We examined this relationship in male roofers with (n=26) or without (n=12) exposure to polycyclic aromatic compounds and female dry cleaners and launderers with (N=18) or without (N=20) exposure to perchloroethylene. For males, urine and blood were obtained pre-shift at the start and post-shift at the end of a work week. For females, urine was obtained pre- and post-shift mid-work week, and blood was obtained once pre-shift mid-work week. 8-OHdG was measured by HPLC-ECD. Linear models were used to look for relationships between leukocyte DNA and urine levels of 8-OHdG. Terms for gender, exposure, smoking, and race were included. Interaction terms were also included to see if the relationship between blood and urine levels varied by these terms. The models were also used to estimate and test individual slopes. For the model comparing pre-shift urine with pre-shift blood, no main effects or interactions were statistically significant, but a significant inverse association (b = -0.1231, p = 0.0336) was noted for the individual sub-group of female controls. For the model comparing pre-shift blood with post shift urine no main effects or interactions or individual slopes were statistically significant. For the model comparing post-shift blood to post shift urine, the smoking interaction was significant (p = 0.0314). Results demonstrate a limited association between leukocyte DNA 8-OHdG and urinary excretion of 8-OHdG.
Urine-chemistry; Roofers; Polycyclic-aromatic-hydrocarbons; Dry-cleaning-industry; Blood-sampling; Sex-factors; Dry-cleaning-solvents; Urinalysis; Analytical-chemistry
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The Toxicologist. Society of Toxicology 41st Annual Meeting and ToxExpo, March 17-21, 2002, Nashville, Tennessee