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Diesel exhaust particle-induced alterations of pulmonary phase I and phase II enzymes of rats.

Authors
Rengasamy-A; Barger-MW; Kane-E; Ma-JKH; Castranova-V; Ma-JYC
Source
J Toxicol Environ Health, A 2003 Jan; 66(2):153-167
NIOSHTIC No.
20022650
Abstract
Although diesel exhaust particles (DEP) are known to produce pulmonary disorders, the xenobiotic metabolic pathways associated with DEP detoxification and bioactivation remain unclear. In this study, the effect of acute exposure of DEP on phase I and phase II enzymes of rat lung was investigated. Intratracheal administration of DEP produced an induction of cytochrome P-450 (CYP) 1A1 enzyme protein and activity at 1 d postexposure, with the enzyme level returning to control at 5 d postexposure. On the other hand, carbon black (CB), a particle control, did not show any induction of CYP1A1 protein or enzyme activity. However, both DEP and CB significantly decreased CYP2B1 protein and enzyme activity at 1 d postexposure. The decrease in CYP2B1 enzyme protein and activity by DEP or CB treatment was observed up to 7 d postexposure. DEP and CB treatments also significantly attenuated glutathione S-transferase (GST)-pi protein at 1 d postexposure. Both DEP and CB at 35 mg/kg significantly decreased the activities of GST and catalase at 1 and 7 d postexposure. DEP, but not CB, significantly induced quinone reductase (QR) activity at 7 d postexposure. This study suggests that DEP may induce CYP1A1 and QR enzymes via a chemical effect, while the carbonaceous core may be involved in the attenuation of CYP2B1, GST, and catalase proteins and enzyme activities.
Keywords
Diesel-exhausts; Pulmonary-disorders; Animal-studies; Animals; Laboratory-animals; Enzymes; Proteins; Exposure-levels; Exhaust-gases; Combustion-gases; Combustion-products; Particulates
Contact
Jane Y. C. Ma, PhD, Pathology and Physiology Research Branch, Health Effects Laboratory Division, NIOSH, 1095 Willowdale Road, Morgantown, WV 26505, USA
CODEN
JTEHD6
Publication Date
20030124
Document Type
Journal Article
Email Address
jym1@cdc.gov
Fiscal Year
2003
NTIS Accession No.
NTIS Price
Issue of Publication
2
ISSN
0098-4108
NIOSH Division
HELD; NPPTL
Priority Area
Work Environment and Workforce: Mixed Exposures
Source Name
Journal of Toxicology and Environmental Health, Part A: Current Issues
State
PA; WV
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