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Hydroxyl radical formation is greater in striatal core than in penumbra in a rat model of ischemic stroke.

Authors
Liu-S; Liu-M; Peterson-S; Miyake-M; Vallyathan-V; Liu-KJ
Source
J Neurosci Res 2003 Mar; 71(6):882-888
NIOSHTIC No.
20022640
Abstract
Although hydroxyl radical ((*)OH) formation has been implicated in the pathophysiological changes of ischemic stroke, (*)OH production in the core and penumbra regions is not clear. It is extremely important to distinguish penumbra from ischemic core in focal cerebral ischemia studies, because the penumbra contains viable tissue, which can be salvaged by appropriate treatment. This study evaluated (*)OH production in both core and penumbra regions of ischemic striatum during ischemia and reperfusion. Microdialysis probes were placed in striatal tissue of rats subjected to the middle cerebral artery occlusion model of ischemic stroke. The (*)OH-trapping agent 4-hydroxybenzoic acid (4-HBA) was administered by both i.v. and probe infusion. Dialysate levels of the 4-HBA oxidation products, 3,4-dihydroxybenzoic acid (3,4-DHBA), were determined by HPLC-ECD. After microdialysis probe delivery of 4-HBA, (*)OH production was significantly increased in the striatal core during both ischemia and reperfusion. Penumbra (*)OH production increased only during reperfusion. Alterations of 3,4-DHBA concentration in dialysate following i.v. 4-HBA administration were likely related to alterations in tissue blood flow. The findings were confirmed by a greater oxidation of dihydroethidium in the ischemic core than in the penumbra as determined by fluorescent microscopy. The findings of (*)OH production in ischemic striatum are the opposite of those reported for ischemic cortex and suggest critical regional variations in (*)OH production that may have significant clinical implications in the treatment of ischemic stroke.
Keywords
Animal-studies; Animals; Laboratory-animals; Models; Blood-samples; Brain-damage; Hydroxyl-groups; Hydroxy-compounds; Cardiovascular-disease
Contact
Ke Jian Liu, PhD, College of Pharmacy, University of New Mexico, 2502 Marble NE, Albuquerque, NM 87131
CODEN
JNREDK
Publication Date
20030315
Document Type
Journal Article
Email Address
jliu@unm.edu
Fiscal Year
2003
NTIS Accession No.
NTIS Price
Issue of Publication
6
ISSN
0360-4012
NIOSH Division
HELD
Priority Area
Work Environment and Workforce: Mixed Exposures
Source Name
Journal of Neuroscience Research
State
NM; WV
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