Evidence for lipopolysaccharide-induced differentiation of RAW264.7 murine macrophage cell line into dendritic like cells.
Saxena-RK; Vallyathan-V; Lewis-DM
J Biosci 2003 Feb; 28(1):129-134
Effect of lipopolysaccharide (LPS) on RAW 264.7 macrophage cell line was studied. LPS-treated RAW264 7 cells increased in cell size and acquired distinct dendritic morphology. At the optimal dose of LPS (1 mg/ml), almost 70% RAW 264.7 cells acquired dendritic morphology. Flow cytometric studies indicate that the cell surface markers known to be expressed on dendritic cells and involved in antigen presentation and T cell acti vation (B 7.1, B 7.2, CD40, MHC class II antigens and CD1d) were also markedly upregulated on LPS-treated RAW 264.7 cells. Our results suggest the possibility that LPS by itself could constitute a sufficient signal for differentiation of macrophages into DC-like cells.
Leukocytes; Bone-marrow; Myeloid-tissue; Antigens; In-vitro-study; Cancer; Morphology
School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India
Journal of Biosciences