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Deficiency in plasma membrane calcium ATPase isoform 2 increases susceptibility to noise-induced hearing loss in mice.

Authors
Kozel-P; Davis-R; Krieg-E; Shull-G; Erway-L
Source
Hear Res 2002 Feb; 164(1-2):231-239
NIOSHTIC No.
20022399
Abstract
Susceptibility to noise-induced hearing loss (NIHL) is poorly understood at the genetic level. Mice homozygous for a null mutation in the plasma membrane Ca2+-ATPase isoform 2 (PMCA2) gene are deaf (Kozel et al., 1998). PMCA2 is expressed on outer hair cell stereocilia (Furuta et al., 1998). Fridberger et al. (1998) observed that the outer hair cell cytoplasmic Ca2+ concentration rises following acoustic overstimulation. We hypothesized that Pmca2+/- mice may be more susceptible to NIHL. Since the auditory brainstem response (ABR) thresholds of Pmca2+/- mice vary with the presence of a modifier locus (Noben-Trauth et al., 1997), Pmca2+/- mice were outcrossed to normal hearing CAST/Ei mice. The pre-exposure ABR thresholds of the resulting Pmca2+/+ and Pmca2+/- siblings were indistinguishable. Groups of these mice were exposed to varying intensities of broadband noise, and ABR threshold shifts were calculated. Fifteen days following an 8 h, 113 dB noise exposure, the Pmca2+/- mice displayed significant (P < or = 0.0007) permanent threshold shifts at 16 and 32 kHz that were 15 or 25 dB greater than those observed in Pmca2+/+ littermates. Pmca2 may be the first gene with a known mutated protein product that confers increased susceptibility to NIHL.
Keywords
Animals; Auditory-system; Enzymology; Noise-induced-hearing-loss; Humans; Genetic-factors
Contact
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, OH 45267, USA.
CODEN
HERED3
Publication Date
20020201
Document Type
Journal Article
Email Address
rrd1@cdc.gov
Fiscal Year
2002
NTIS Accession No.
NTIS Price
Issue of Publication
(1-2)
ISSN
0378-5955
NIOSH Division
DART
Source Name
Hearing Research
State
OH
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